Sino Biological provides an extensive range of recombinant cytokines specifically designed for cell culture to promote research in tumor immunotherapy, stem cell treatment, drug screening, and regenerative medicine. Through rigorous quality control and extensive validation, the company ensures that cytokines have minimal endotoxin levels, high stability, proven biological activity, and high purity.
Recombinant cytokines, which support a variety of cell types, such as stem cells, brain cells, immune cells, and organoids, are available for several species, including humans, rats, mice, and more. This helps to speed up scientific findings and facilitate functional investigations.
Sino Biological is dedicated to producing top-notch clinical research and medication development reagents. In addition to RUO-grade cytokines, a range of GMP-grade cytokines that exhibit superior stability and quality have been created through the application of a GMP quality management system. These cytokines have comprehensively supported cell therapy and drug development processes.
Product advantages
- 250+ molecules, 800+ cytokine products (20+ GMP-grade)
- Superior purity and lot-to-lot uniformity
- Comprehensive coverage, including interleukins, growth factors, TNFs, chemokines, interferons, and more
- Elevated biological activity
- To cover a variety of animal models, species such as humans, mice, monkeys, and others
- Little or no tags
- Carrier free
- Low amounts of endotoxins
- Grades GMP and RUO are accessible
- Certifications for quality systems: ISO9001, ISO13485, and GMP
A wide range of applications
GMP-grade cytokines—including IL-2, IL-7, IL-15, and IL-21—completely support the production of medications used in cell therapy.
Growth factors, such as PDGF-BB, EGF, and FGF2, are better suited for the in vitro culture, proliferation, and differentiation of stem cells, such as MSCs and iPSCs.
Numerous organoid platforms clients employ have proven the quality and efficacy of recombinant growth factors, which have earned a widespread reputation in the industry.Inc.
Premium target proteins cover all of the popular cytokine targets, facilitating important processes such as antibody screening, affinity testing, and vaccination.
Product validation data
GMP-grade cytokines: Complete quality release verification
The GMP-grade Human IL-21 (Cat#: GMP-10584-HNAE) is a prime example of dedication to excellence. Produced using stringent GMP quality control procedures, every batch is tested extensively using Chinese and American Pharmacopoeia techniques.
These tests guarantee adherence to strict criteria and cover various topics, including purity, endotoxin, mycoplasma, sterility, and more. This product is defined by high activity, stability, and lot-to-lot consistency.
Safety release testing
- Bacterial endotoxin: <5–10 EU/mg
- Host cell DNA: <100 pg/mg
- Host cell protein: <0.5 μg/mg (500 ppm)
- Human-derived virus: Negative
- Mycoplasma: Negative
- Sterility: No Growth
- Abnormal toxicity: All alive and healthy
Freeze-thaw stability
Three and five cycles of freeze-thaw tests were conducted to verify GMP-grade human IL-21 (Cat#: GMP-10584-HNAE) stability. Image Credit: Sino Biological US Inc.
Accelerated stability
GMP-grade human IL-21 (Cat#: GMP-10584-HNAE) was stable at 25 °C for 16, 24, and 48 days. Image Credit: Sino Biological US Inc.
High lot-to-lot consistency
Two independent lots of GMP-grade human IL-21 (Cat#: GMP-10584-HNAE) showed identical bioactivity. Image Credit: Sino Biological US Inc.
IL-2, IL-15, and IL-21 in combination to induce NK cell differentiation
Human IL-2, IL-15, and IL-21 (Cat#: 11848-HNAH1-E; Cat#: 10360-HNCE; Cat#: 10584-HNAE)
Natural killer (NK) cells expansion was detected by FACS analysis. Representative graphs of CD3−CD56+ (CD56bright and CD56dim) expressing cells demonstrated 3-day treatment of 20 ng/mL IL-2, 20 ng/mL IL-15, and 25 ng/mL IL-21 induced NK cells expansion. Image Credit: DOI: 10.1155/2023/8924603
Excellent performance validated by multiple organoid cultures
Human breast cancer organoids
Human breast cancer organoids were cultured with FGF7 (Cat#: 10210-H07E), RSPO1 (Cat#: 11083-HNAS), IGF1 (Cat#: 10598-HNAE), EGF (Cat#: 50482-MNCH), NRG1 Beta 1 (Cat#: 11609-H01H), and NOG (Cat#: 50688-M02H). Image Credit: Sino Biological US Inc.
Human liver cancer organoids
Human liver cancer organoids were cultured with FGF2 (Cat#: 10014-HNAE), HGF (Cat#: 10463-HNAS), FGF7 (Cat#: 10210-H07E), EGF (Cat#: 50482-MNCH), FGF10 (Cat#: 10573-HNAE), TGFB1 (Cat#: 10804-HNAC), NOG (Cat#: 50688-M02H), and RSPO1 (Cat#: 11083-HNAS). Image Credit: Sino Biological US Inc.
Human biliary cancer organoids
Human cholangiocarcinomas organoids were cultured with FGF2 (Cat#: 10014-HNAE), HGF (Cat#: 10463-HNAS), FGF7 (Cat#: 10210-H07E), EGF (Cat#: 50482-MNCH), FGF10 (Cat#: 10573-HNAE), NOG (Cat#: 50688-M02H), and RSPO1 (Cat#: 11083-HNAS). Image Credit: Sino Biological US Inc.
Human colorectal organoids
Human colorectum organoids were cultured with RSPO1 (Cat#: 11083-HNAS), EGF (Cat#: 50482-MNCH), and NOG (Cat#: 50688-M02H). Image Credit: Sino Biological US Inc.
Comprehensive QC testing methods to protect product quality
Verified by SEC-HPLC
Recombinant Mouse IL-23 Heterodimer Protein, His Tag, HPLC-verified (Cat#: CT140-M08H) was more than 95% as determined by SEC-HPLC. Image Credit: Sino Biological US Inc.
Verified by SEC-MALS
Recombinant Human IL17A & IL17F Heterodimer Protein, Strep II & His-AVI Tag, HPLC-verified (Cat#: CT141-H8156H-B) was more than 95% and the molecular weight of this protein is around 39.7 kDa verified by SEC-MALS (Routinely tested). Image Credit: Sino Biological US Inc.
Verified by ELISA
Immobilized mouse CSF1 (Cat#: 51112-MNAH) at 2 μg/ml (100 μl/well) can bind mouse CSF1R-Fch. Image Credit: Sino Biological US Inc.
Verified by SPR
Captured Adalimumab on Anti-human IgG Fc via CM5 Chip can bind TNF-α (Cat#: 10602-HNAE) with an affinity constant of 0.2843 nM, as determined in an SPR assay (Biacore T200) (routinely tested). Image Credit: Sino Biological US Inc.
In the past, cytokines and their receptors have been critical therapeutic targets for the treatment of inflammatory, autoimmune, and cancer disorders. Sino Biological produces an extensive range of recombinant cytokines and growth factors, including interleukins, growth factors, interferons, and chemokines, with great success. Sino Biological accelerates the development of anti-cytokine medication by offering vital support. In addition, Sino Biological also provides custom cytokine protein development services.