The Transcreener cGAMP cGAS Assay relies on direct detection of cGAMP produced by cGAS to measure enzyme activity. The assay uses antibodies that selectively bind cGAMP in the presence of excess ATP and GTP. When the fluorescent tracer is displaced by cGAMP, a change in signal is achieved. The novel direct detection method simplifies the protocol (no coupling enzyme needed) and minimizes compound interference.
The assay can be used to:
- Quantify Inhibitor Potency
- Determine Inhibitor-cGAS Residence Time
- Measure Enzymatic Activity of cGAS
- Screen Compound Libraries for cGAS inhibitors
Image Credit: BellBrook Labs
Why Choose Transcreener?
- Direct detection of unlabeled cGAMP
- Easy to use and HTS ready
- Robust Assay with Z’ > 0.7
- Far-red fluorescent readouts reduce compound interference
- A non-radioactive, safe technique
- Available in FP or TR-FRET readout depending on preference and plate reader compatibility
Example cGAS Data
24+ Hour Signal Stability for HTS
cGAS titration at 1 hour and 24 hours demonstrates the assay's oustanding signal stability. The signal stability seen here is crucial for HTS where the screen size can require significant more time.
Image Credit: BellBrook Labs
Detecting cGAMP Under Initial Velocity Conditions
cGAS titration using Transcreener cGAMP cGAS Assay with an FP readout.
Image Credit: BellBrook Labs
cGAS linear response. The assay demonstrates linearity when raw data is converted to cGAMP using a standard curve.
Image Credit: BellBrook Labs
Screen Compound Libraries & Perform Dose Responses
cGAS dose response with known cGAS inhibitor, G150, using Transcreener cGAMP FP Assay. IC50 (G150)= 0.04 μM.
Image Credit: BellBrook Labs
Lead Discovery Services
BellBrook Labs also offers lead discovery services for scientists who want to advance their program but don't want to bring a biochemical assay in-house. Their services feature customizable assay conditions, quick turnaround times, and direct collaboration with a BellBrook scientist. Lead discovery services can include:
- Inhibitor Screening
- Inhibitor Potency Profiling
- Inhibitor Selectivity Profiling
- Residence Time Measurements
- Mechanism of Action Studies
- Triage Non-Stoichiometric Inhibitors
- Compound-Target Binding with Thermal Shift Assays