CyFlow Ploidy Analyser from Partec

Automatic Measurement of Ploidy Level and Aneuploidy in Plants and Animals

Ploidy determinations with the CyFlow® Ploidy Analyser are performed by the precise measurement of the total DNA content of individual nuclei extracted and stained with Partec CyStain® reagents, which can be derived from almost any plant or animal tissue. When used in combination with Partec CyStain® PI reagents, the relative genome size of plant and animal nuclei can also be determine on the CyFlow® Ploidy Analyser.
 
The CyFlow® Ploidy Analyser measures the DNA content of many thousands of individual nuclei within seconds or minutes. The result is displayed as a DNA histogram in real time on an 8” colour touchscreen together with the ploidy level. The automated, real-time ploidy analysis is extremely time- and cost- efficient.
 
Highlights
 
  • Ploidy measurement in less than two minutes
  • Detection of Anisoploids, Allopolyploids and Aneuploids
  • Plant Genome Size and C-Value Determination
  • Screening for Haplo-, Diplo-, Triplo- and Polyploids
  • Detection with +/- 1 chromosome accuracy in most plants
  • Detection of Apomixis and Reproduction Behaviour
  • Plant Genome Size and C-Value Determination
  • Cytotype Identification in Natural Populations
  • Hybrid Detection
  • Polysomaty and Chimeric Plants Analysis
  • Sex Detection – Nuclear Base Composition
  • Preparation and staining of metaphase chromosomes is not necessary
  • No time-consuming microscopic evaluation; no chromosome counting
  • Easy sample preparation by ready-to-use protocols
  • Propidium Iodide and DAPI based DNA fluorescence
  • All tests on leaf, root or callus material
  • Perfect for screening of In-Vitro plant material
  • Early stage tests on seeds and seedlings
  • Any plant material can be analysed: leaves, seedlings, roots, flowers, peel of fruits, seeds

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