Immunology researchers at UT Southwestern Medical Center have found that bacteria present in the human gut help initiate the body's defense mechanisms against Toxoplasma gondii, the parasite responsible for toxoplasmosis.
Toxoplasmosis is generally a mild infection, but it can have serious and potentially fatal effects in pregnant women, their fetuses and others with weakened immune systems.
In mice, T gondii directly activates a specific immune protein in the host, called toll-like receptor 11 (TLR-11), which helps control the animals' immune response to the parasite. Humans, however, don't have an active form of this receptor. Exactly how the body senses T gondii has remained unclear because the parasite doesn't activate any of the functioning toll-like receptors that humans do possess.
In a new study appearing online and in the Aug. 20 issue of Cell Host & Microbe, researchers at UT Southwestern suggest that instead of activating toll-like receptors directly, T gondii's first interaction in the human gut is with the helpful bacteria that live inside us. Those bacteria then release signaling molecules, alerting the human host to the invader.
"While this is very early data, our results suggest that looking at the bacteria present in each patient's gut could help physicians understand their susceptibility to infectious diseases," said Dr. Felix Yarovinsky, assistant professor of immunology at UT Southwestern and senior author of the paper. "It also suggests the possibility of developing novel probiotic strategies for treating parasitic infections such as toxoplasmosis and cryptosporidiosis, a related disease caused by the parasite Cryptosporidium."
T gondii affects more than 1 billion people worldwide. The protozoan parasite can infect most warm-blooded animals, but the primary host is the house cat. Animals are generally infected with T gondii by ingesting contaminated meat, water or the feces of a cat that has recently been infected; however, the parasite also can be passed from mother to fetus.
Because toxoplasmosis is passed to humans through contaminated cat feces, pregnant women are encouraged to keep all house cats indoors and recruit someone who is not pregnant to clean the litter box daily. Once a person is infected, the parasite penetrates the intestine and spreads throughout all organs.
The researchers studied mice in which TLR-11 had been genetically eliminated. This mimics the human immune response to T gondii. They then infected the TRL-11-deficient mice with T gondii both orally and systemically by injection.
Even though the mice lacked their normal mechanism for fighting infection, they nonetheless mounted an attack against T gondii. The researchers found that the commensal - or good - bacteria in the gut activated their immune system, thereby inducing various inflammatory responses against the invading pathogen. In humans, he said, it is those helpful bacteria that send activating signals to the three toll-like receptors that are functional, inducing various inflammatory responses against invading pathogens like T gondii.