A variant of last year's pandemic influenza linked to fatal cases carried a mutation that enabled it to infect a different subset of cells lining the airway, according to new research. The study, due to be published next week in the Journal of Virology, suggests that the mutant virus could have impaired the lungs' ability to clear out germs. The researchers behind the study, from Imperial College London, the Medical Research Council National Institute for Medical Research and the University of Marburg said the findings highlight the potential for deadlier strains of flu to emerge and spread.
The 2009 pandemic of H1N1 influenza caused thousands of deaths worldwide, but the majority of cases were relatively mild. A variant of the virus carried a mutation termed D222G in a protein on the surface of the virus, and people infected with this variant were more likely to have severe and fatal illness. According to a World Health Organisation report, the D222G mutation was found in less than two in every hundred cases of 2009 pandemic flu, but was responsible for around seven in every hundred deaths.
Viruses infect cells by attaching to receptor molecules on the cell surface. Different receptors are present on different cell types, and a virus can only infect cells that have the right receptors for the protein on its own surface.
The new research shows that flu virus with the D222G mutation can bind to a broader range of receptors in the airway, including receptors that are present on cells called ciliated cells. These cells, found in the lining of the airway, have hair-like projections called cilia. The cilia sway back and forth to move mucus with trapped particles upward toward the mouth, and this is normally swallowed or coughed up. When ciliated cells become infected, the cilia stop moving and this vital clearance function is impaired. Inhaled viruses and bacteria can then reach the lung more easily, where they can potentially cause pneumonia.
The mutant virus has an increased capacity to infect ciliated cells, as shown by the collaborating group at the University of Marburg. Infection of the ciliated cells would sabotage the lungs' clearing mechanism and could be one factor that made the D222G mutation more virulent, the researchers suggest.
"This simple mutation, which swapped one building block of a virus protein for another, apparently resulted in a more virulent version of the H1N1 virus," said Professor Ten Feizi from the Department of Medicine at Imperial College London, who led the study. "We think this is at least partly due to the virus being able to bind to different receptors, which allowed it to infect ciliated cells and stop them from clearing out germs.