Scientists develop new tool for analyzing and characterizing aspects of the brain's neural circuitry

Published on June 17, 2013 at 2:09 AM · No Comments

Discovery provides model system for studying how the brain integrates multiple stimuli and initiates motor response

Scientists at the University of Massachusetts Medical School have developed a novel transgenic system which allows them to remotely activate individual brain cells in the model organism Drosophila using ambient temperature. This powerful new tool for identifying and characterizing neural circuitry has lead to the identification of a pair of neurons - now called Fdg neurons - in the fruit fly that decide when to eat and initiate the subsequent feeding action. Discovery of these neurons may help neurobiologists better understand how the brain uses memory and stimuli to produce classically conditioned responses, such as those often associated with phobias or drug tolerance. The study appears in the journal Nature.

"For any organism, the decision to eat is a complex integration of internal and external stimuli leading to the activation of an organized sequence of motor patterns," said Motojiro Yoshihara, PhD, assistant professor of neurobiology at the University of Massachusetts Medical School and lead author of the Nature study. "By developing genetic tools to remotely activate individual brain cells in Drosophila, we've been able to isolate a pair of neurons that are critical to the act of eating in fruit flies. More importantly, we now have a powerful new tool with which we can answer important questions about the function and composition of neural circuitry."

To isolate the neurons responsible for sensing food and initiating the complex feeding program in Drosophila, UMMS scientists had to develop a method of studying the behavior of freely moving flies while targeting and manipulating individual neurons. To accomplish this, Dr. Yoshihara expressed temperature activated genes in random neurons in more than 800 Drosophila lines. Placing these genetically modified flies in a small temperature-controlled chamber, he was able to active these genes by increasing and decreasing the ambient temperature. This, in turn, activated the corresponding neurons.

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