Peptide Synthesis

Published on May 15, 2013 at 11:07 PM

Custom manufactured peptides are in high demand for immunological studies and require highly accurate quality control, fast delivery and cost effectiveness.

Most researchers require exact purity, specified quantity and specialist modifications for their research needs.

AMSBIO provides a full range of:-

  • custom peptide products
  • peptide microarrays
  • peptide libraries
  • simple to complex peptides
  • linear to cyclised peptides
  • large to small scale production
  • long sequence peptides (up to 140 amino acid in length).

Variety of modifications

AMSBIO offer a variety of modifications like biotinylation, phosphorylation, acetylation, dye labelling, cyclic peptides and MAPS.

MAPs or Multi-Antigenic Peptides are branched peptides where the linear peptide chains are linked at their C-terminus via polylysine core. This increases the molecular size.

Purity levels

AMSBIO offer a wide range of purity levels (desalted, >75%, >85%, >90%, >95%, >98% and even above 99%) with HPLC purifications (5 - 20 mg scale).

Purity levels of >75% to >85% are recommended for immunological studies, polyclonal antibody productions.

Purity levels of more than 90% are needed for bioassays while in vitro bioassays such as ELISA and enzyme studies require >95% purity.

When doing structural studies like sensitive bioassays, NMR and crystallography purity of over 98% is commonly requested.

How are the peptides developed?

The peptides are developed using BLAST databases to search for homology to the protein of interest and select the best region for the synthesis of the desired peptide.

AMSBIO also provides protein antigenicity analysis and region hydrophilicity analysis and uses best amino acid coupling efficiency to decide design strategy. Customers receive advice in design when considering important questions such as KLH coupling and cross species reactivities.

AMSBIO also provide related peptides and peptidomimetics for T-cell receptor, epitope mapping studies and drug discovery.

All deliveries of peptides from AMSBIO are supplied with documentation including HPLC chromatogram, mass spectrophotometric analysis and synthesis report.

Source

http://www.amsbio.com/custompeptidesynthesis.aspx


AMSBIO is a premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.

Last updated: Aug 6, 2013 at 7:04 AM

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