2019-nCoV NSP10/NSP16 Methyltransferase, Active

A number of eukaryotic viruses have evolved 2′-O-methyltransferases (2′-O-MTase) to alter their viral mRNAs and carry a cap-1 structure (m7GpppNm) at the 5′ end.

This 5' cap structure is crucial for the stability of viral mRNA, viral immune escape, and protein translation (1). SARS-CoV has NSP16 that shows 2′-O-MTase activity. NSP16 needs NSP 10 for stimulation, which is a conserved activity in coronaviruses (2).

Therefore, inhibitors aiming at the NSP10/NSP16 2′-O-MTase are promising targets for designing anti-coronavirus drugs (3).

Product specifications

Specific activity

Sample Activity Plot.

Sample Activity Plot. Image Credit: SignalChem Biotech

For particular data on a given lot, users can refer to the related technical data sheet.

Purity

Sample Purity Data.

Sample Purity Data. Image Credit: SignalChem Biotech

For particular data on a given lot, users can refer to the related technical data sheet.

Assay Data

Storage, stability, and shipping

The product should be stored at –70 °C. For optimal storage, the target can be aliquoted into smaller quantities after centrifugation and then stored at the suggested temperature. For best performance, multiple freeze/thaw cycles and frequent handling should be avoided.

Molecular weight

19 kDa (NSP10) and 38 kDa (NSP16)

References

  1. Furuichi, Y. et al: Viral and cellular mRNA capping: past and prospects. Adv Virus Res. 2000, 55:135–184.
  2. Decroly, E. et al. Coronavirus nonstructural protein 16 is a cap-o binding enzyme possessing (nucleoside-2'O)-methyltransferase activity. J Virol. 2008, 82(16):8071-8084.
  3. Chen, Y. et al: Biochemical and structural insights into the mechanisms of SARS coronavirus RNA ribose 2’-O-methylation by nsp16/nsp10 protein complex. PLoS Pathog. 2011, 7(10): e1002294.