Anti 2019-nCoV Spike Protein Llama Heavy-Chain Antibody Catalog No: C19S1-641HL

The recombinant Llama VHH-Human IgG Antibody from SignalChem Biotech is a high-sensitivity SARS-CoV-2 spike protein detection assay. The particular activity was quantified using a couple of Functional ELISA systems, and this was done by testing the binding ability of the nanobody to SARS-CoV-2 spike protein.

Scientific background

SARS-CoV-2—the severe acute respiratory syndrome-related novel coronavirus—has been responsible for causing the pandemic of respiratory diseases (COVID-19) throughout the world in 2020.1

The spike glycoprotein (S) of coronavirus is part of the type I transmembrane protein that contains two subunits, S1 and S2.2 These subunits are also known to be the main components to attach to host cells via the interaction with angiotensin-converting enzyme 2 (ACE2).3

A receptor binding domain (RBD) of S1 is capable of identifying the cell surface receptor, while the RBD mutation could result in higher motility rate.3

Specific activity

The binding ability measured in a functional ELISA. Immobilized Anti 2019-nCoV Spike Protein Llama heavy-chain (C19S1-641HL) binds 2019-nCoV Spike protein RBD (C19SD-241H) that can be detected at picomol level using Anti 2019-nCoV Spike Protein hIgG Antibody (C19S1-61H).

The binding ability measured in a functional ELISA. Immobilized Anti 2019-nCoV Spike Protein Llama heavy-chain (C19S1-641HL) binds 2019-nCoV Spike protein RBD (C19SD-241H) that can be detected at picomol level using Anti 2019-nCoV Spike Protein hIgG Antibody (C19S1-61H). Image Credit: SignalChem Biotech Inc.

The binding of Anti 2019-nCoV Spike Protein Llama heavy-chain (C19S1-641HL) to 2019-nCoV RBD (C19SD-G241H) determined by ELISA.

The binding of Anti 2019-nCoV Spike Protein Llama heavy-chain (C19S1-641HL) to 2019-nCoV RBD (C19SD-G241H) determined by ELISA. Image Credit: SignalChem Biotech Inc.

References

  1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270–89.
  2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428–30.
  3. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235.