The BX51WI is ideal for all physiological experiments such as patch clamping and intravital microscopy. The fixed stage concept and vibration-free frame design ensure excellent stability throughout the experiment. Use of infrared light protects living cells and offers high penetration depths of thick tissue slices, while high NA optics allow magnification changes without moving the objective.
The BX61WI is the motorized version of the BX51WI fixed-stage microscope with a highly accurate Z-drive. It is the ideal tool for all automated physiological experiments, such as patch clamping and intravital microscopy.
Vibration-free Magnification Changes
A major concern for researchers conducting electrophysiology experiments is the vibration which occurs when switching objectives and the resulting interference this can cause to the specimens and adjacent equipment.
To solve this problem, Olympus introduces a concept - the provision of an intermediate magnification changer in combination with the High NA long working distance 20x objective that allows the user to switch between low and high magnifications without the need to switch objectives.
IR-DIC observation, trigeminal motor neuron, Tomio Inoue Ph.D, Department of Oral Physiology, Showa University
Safe Magnification Changes
The 20x water immersion objective (XLUMPLFLN20xW) makes high-resolution observation possible with a wide range of intermediate magnification lenses. Since exchanges between low and high magnification are performed through the intermediate magnification changer, vibration is reduced to a minimum and the usual concern about collisions between objectives and patch clamp electrodes is eliminated.
Simultaneous Fluorescence and IR-DIC Imaging
With the included 690 nm dichroic mirror in the WI-DPMC, fluorescence light is sent to the front port, and IR-DIC light is sent to the back port allowing two cameras to image simultaneously with no vibration introduced by light path selection. IR-DIC observation is compatible with 775 nm and 900 nm wavelengths.
Magnification Selector with Minimal Vibration
The WI-DPMC rear camera port includes a 2 position intermediate magnification selector. A high magnification 4x intermediate lens is included and a (0.25x or 0.35x) low magnification lens is optional.
High or low magnification selection is via a single lever with no click-stops or detents allowing a specimen to be scanned and measured with minimal disturbance from vibration.
*Available for 0.5x, 1x and 2x intermediate magnification lenses by special order.
Variable Click-stops with Minimal Vibration
All click-stops, as when selecting between camera and observation modes, can be adjusted to have no click and thus no vibration.