The processes of buffer exchange and sample concentration are laborious and need a lot of manual work. The Big Tuna from Unchained Labs offers an automated solution for exchange to formulate, concentrate and clean up proteins or gene therapy vectors like LNPs, VLPs and AAVs.

Just 30 minutes of setup time would be sufficient to execute these processes. The Big Tuna avoids the need for slow and manual methods of making samples, allowing users to dedicate their time to other crucial work.

Listed below are the several sample types:

  • LNP
  • VLP
  • AAV
  • Protein
  • mRNA and DNA
Unchained Tackles Science in 5 - Buffer Exchange with Big Tuna

Video Credit: Unchained Labs

Buffer exchange in 24 or 96 well formats

Big Tuna has the ability to use two plate-based formats that allow researchers to exchange and concentrate 24 or 96 samples at the same time. The system comprises Unfilter 96 for users to exchange as little as 100 μL for up to 96 different samples in a single run.

Unfilter 24 allows users to execute huge exchanges of as much as 8 mL on up to 24 samples. It comes with a range of molecular weight cutoff options to guarantee that users have the right Unfilter for their experiment.

Big Tuna: The ultimate automated buffer exchange solution

Image Credit: Unchained Labs

Hands-off, automated buffer exchange

Big Tuna’s unique pressure-based UF/DF process and gentle mixing enable it to maintain buffer exchange in a uniform manner throughout the plate. The acoustic sensor of the system can quantify the volume in each well after each cycle to measure the difference in sample exchange rates.

Then, Big Tuna evaluates and adds back the amount of buffer required to make it even before the next cycle. The process is in progress until the programmed exchange and concentration target has been reached.

Big Tuna: The ultimate automated buffer exchange solution

Image Credit: Unchained Labs

Concentrate dilute samples

Dilution of samples is a tedious process. The Big Tuna has the ability to concentrate 48 mL down to 8 mL for up to 24 samples at the same time. Users can track buffer exchange to readily take the AAVs and other high-volume, low-concentration samples to where they are easily accessible freely.

Big Tuna: The ultimate automated buffer exchange solution

Image Credit: Unchained Labs

Exchange your samples exactly how you want

It is not necessary for buffer exchange to be a shot in the dark or to keep the user anchored to the bench across the entire process. The software wizard makes use of drag and drop for an operator to intuitively position the samples and buffers into place.

Guidance is offered for users through all the methods for tailoring the run for a particular sample type. The simple setup enables users to have more control over the experiment with minimal hands-on time.

Big Tuna: The ultimate automated buffer exchange solution

Image Credit: Unchained Labs

Check in on progress

The Big Tuna is a trustworthy system for processing samples. The system takes users through the setup process before they leave. Users can check in on the progress of operations at any time and view data concerning the time remaining for exchange and concentration runs. The system does not need manual monitoring and frees up time to enable users to handle other tasks.

Big Tuna: The ultimate automated buffer exchange solution

Image Credit: Unchained Labs