Cancer Cell Line Medium for In Vitro Cultivation

The PromoCell xeno-free medium is used for the standardized in vitro cultivation of human cancer cell lines.

Description

The PromoCell Cancer Cell Line Medium XF is a cell culture medium, created for the standardized in vitro cultivation of human cancer cell lines.

Its xeno-free and serum-free formulation provides a culture setting devoid of all stimuli emanating from non-defined materials. Therefore, it has no ill-defined constituents (such as extracts, fetal calf serum, or hydrolysates) and reveals very low lot-to-lot variability.

Components

The PromoCell Cancer Cell Line Medium XF includes a bottle of Basal Medium and one vial of SupplementMix. Incorporating the SupplementMix to the Basal Medium creates the complete medium.

Note: Culture vessels have to be coated. For coating, it is proposed that Human Fibronectin Solution (C-43060) or Vitronectin (C-69201) is used.

Key Features

  • Xeno-free and serum-free formulation
  • Ideal for long-term routine culture of human cancer cell lines
  • Adheres with most commonly used human cancer cell lines

The following table provides a list of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:

Tissue Tested Cell Lines Cell Lines Origin Remarks
Brain BV2 immortalized murine primary microglial cells Coat with Fibronectin: 1 µg/cm2
Breast MCF-7 pleural effusion of metastatic human breast adenocarcinoma Coat with Fibronectin: 1 µg/cm2
Colon HT-29 human colon adenocarcinoma Coat with Vitronectin: 0.5 µg/cm2
Connective tissue HT1080 human fibrosarcoma Coat with Fibronectin: 1 µg/cm2
Liver HepG2 hepatocellular carcinoma of the human liver Coat with Vitronectin: 0.5 µg/cm2
Lung A-549 human lung carcinoma Coat with Vitronectin: 0.5 µg/cm2
Prostate LNCaP lymph node metastasis of human prostate adenocarcinoma 3D culture in C-28070 is recommended