The PromoCell xeno-free medium is used for the standardized in vitro cultivation of human cancer cell lines.
Description
The PromoCell Cancer Cell Line Medium XF is a cell culture medium, created for the standardized in vitro cultivation of human cancer cell lines.
Its xeno-free and serum-free formulation provides a culture setting devoid of all stimuli emanating from non-defined materials. Therefore, it has no ill-defined constituents (such as extracts, fetal calf serum, or hydrolysates) and reveals very low lot-to-lot variability.
Components
The PromoCell Cancer Cell Line Medium XF includes a bottle of Basal Medium and one vial of SupplementMix. Incorporating the SupplementMix to the Basal Medium creates the complete medium.
Note: Culture vessels have to be coated. For coating, it is proposed that Human Fibronectin Solution (C-43060) or Vitronectin (C-69201) is used.
Key Features
- Xeno-free and serum-free formulation
- Ideal for long-term routine culture of human cancer cell lines
- Adheres with most commonly used human cancer cell lines
The following table provides a list of cell types tested for serial passage with the PromoCell Cancer Cell Line Medium XF:
| Tissue |
Tested Cell Lines |
Cell Lines Origin |
Remarks |
| Brain |
BV2 |
immortalized murine primary microglial cells |
Coat with Fibronectin: 1 µg/cm2 |
| Breast |
MCF-7 |
pleural effusion of metastatic human breast adenocarcinoma |
Coat with Fibronectin: 1 µg/cm2 |
| Colon |
HT-29 |
human colon adenocarcinoma |
Coat with Vitronectin: 0.5 µg/cm2 |
| Connective tissue |
HT1080 |
human fibrosarcoma |
Coat with Fibronectin: 1 µg/cm2 |
| Liver |
HepG2 |
hepatocellular carcinoma of the human liver |
Coat with Vitronectin: 0.5 µg/cm2 |
| Lung |
A-549 |
human lung carcinoma |
Coat with Vitronectin: 0.5 µg/cm2 |
| Prostate |
LNCaP |
lymph node metastasis of human prostate adenocarcinoma |
3D culture in C-28070 is recommended |