The Cell-Based Cholesterol Efflux Fluorometric Assay Kit from BioVision.
Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is a preliminary and critical step in Reverse Cholesterol Transport (RCT). RCT is the procedure by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, carry unnecessary cholesterol back to the liver for bile acid synthesis and excretion, thereby lowering the peripheral lipid burden. A negative correlation has been confirmed between the in vitro efflux of cholesterol from atherosclerosis and macrophages. BioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay offers a safe, sensitive, and reproducible technique for measuring cholesterol efflux.
|Cat # +Size
|Fluorescence (Ex/Em = 482/515 nm).
||Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux.
Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).
|Features & Benefits
- Simple & Rapid Protocol
- Convenient: Non-Radioactive, no special handling or disposal required
- Accurate: reproducible results with low intra & inter assay variability
- Labeling Reagent
- Equilibration Buffer
- Reagent A
- Reagent B
- Cell Lysis Buffer
- Positive Control
- Serum Trea™ent Reagent
||For research use only! Not for use in humans