Cell-Based Cholesterol Efflux Fluorometric Assay Kit

The Cell-Based Cholesterol Efflux Fluorometric Assay Kit from BioVision.

Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is a preliminary and critical step in Reverse Cholesterol Transport (RCT). RCT is the procedure by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, carry unnecessary cholesterol back to the liver for bile acid synthesis and excretion, thereby lowering the peripheral lipid burden. A negative correlation has been confirmed between the in vitro efflux of cholesterol from atherosclerosis and macrophages. BioVision’s Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay offers a safe, sensitive, and reproducible technique for measuring cholesterol efflux.

Product Details

Cat # +Size K582-100
Size 100 assays
Detection
Method
Fluorescence (Ex/Em = 482/515 nm).
Applications Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux.
Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).
Features & Benefits
  • Simple & Rapid Protocol
  • Convenient: Non-Radioactive, no special handling or disposal required
  • High-Throughput
  • Accurate: reproducible results with low intra & inter assay variability
Kit Components
  • Labeling Reagent
  • Equilibration Buffer
  • Reagent A
  • Reagent B
  • Cell Lysis Buffer
  • Positive Control
  • Serum Trea™ent Reagent
Storage Conditions -20 °C
Shipping Conditions Gel Pack
Usage For research use only! Not for use in humans