The Colorimetric Assay Kit from BioVision comprises of non-radioactive, highly sensitive assay.
Glucose uptake is a vital biological process for examining cell signaling and glucose metabolism. Among a number of different approaches available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been extensively used due to its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be additionally metabolized, and thus collects in the cells. The collected 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells.
In BioVision’s glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to yield NADPH, which can be established by an enzymatic recycling amplification reaction. This user-friendly non-radioactive kit is extremely sensitive and can detect glucose uptake as low as 10 pmol/well.
|Cat # +Size
|Absorbance (412 nm)
||This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.
|Features & Benefits
- Simple procedure
- Fast and convenient
- The assay is easy, non-radioactive, and highly sensitive
- Extraction Buffer
- Neutralization Buffer
- 2-Deoxyglucose (2-DG, 10 mM)
- Assay Buffer
- Enzyme Mix (lyophilized)
- Recycling Mix (lyophilized)
- 2-DG6P Standard (lyophilized)
- Glutathione Reductase (lyophilized)
- Substrate-DTNB (lyophilized)
||For research use only! Not for use in humans