Colorimetric Assay Kit for Glucose Uptake

The Colorimetric Assay Kit from BioVision comprises of non-radioactive, highly sensitive assay.

Glucose uptake is a vital biological process for examining cell signaling and glucose metabolism. Among a number of different approaches available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been extensively used due to its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be additionally metabolized, and thus collects in the cells. The collected 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells.

In BioVision’s glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to yield NADPH, which can be established by an enzymatic recycling amplification reaction. This user-friendly non-radioactive kit is extremely sensitive and can detect glucose uptake as low as 10 pmol/well.

Product Details

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Cat # +Size K676-100
Size 100 assays
Detection
Method
Absorbance (412 nm)
Species
Reactivity
Mammalian
Applications This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.
Features & Benefits
  • Simple procedure
  • Fast and convenient
  • The assay is easy, non-radioactive, and highly sensitive
Kit Components
  • Extraction Buffer
  • Neutralization Buffer
  • 2-Deoxyglucose (2-DG, 10 mM)
  • Assay Buffer
  • Enzyme Mix (lyophilized)
  • Recycling Mix (lyophilized)
  • 2-DG6P Standard (lyophilized)
  • Glutathione Reductase (lyophilized)
  • Substrate-DTNB (lyophilized)
Storage Conditions -20 °C
Shipping Conditions Gel Pack
Usage For research use only! Not for use in humans