Corning® HYPERStack® Vessels: a closed system for high-yield cell growth

The HYPERStack cell culture vessel incorporates the best features of two Corning products: the CellSTACK® and HYPERFlask® cell culture vessels.

The incorporation of gas-permeable film technology provided in the footprint of a CellSTACK vessel makes the HYPERStack platform among the most efficient, scalable adherent cell culture vessels available today.

Features and benefits

  • Scalable product — Scale-up and scale-out are supported by multiple size offerings
  • Fixed media volume — 0.2 mL/cm2 fills vessel for less volumetric waste
  • More cells — Delivers up to 5× the growth surface area of a typical cell culture vessel of comparable dimensions
  • Ergonomic design — Easier manipulation with handling equipment and accessories
  • Closed system — No open fluid manipulations

Corning® HYPERStack®-a closed system for high-yield cell growth

Image Credit: Corning Life Sciences

Corning® HYPERStack® cell culture vessels improve scale-up efficiency

(A) Final expansion of Human Mesenchymal Stem Cells in Corning HYPERStack 12-layer or control T-flask cell culture vessels with Corning CellBIND® surface treatment using hMSC medium. (B) Total Corning HYPERStack vessel yields of Vero cell cultures were 4X greater than yields from Corning CellSTACK® vessels with similar cubic footprint. (C) Corning HYPERStack vessels generated a significantly higher amount of total infectious adenoviral particles versus stacked vessels of similar cubic footprint. Total infectious adenoviral particles were calculated based on titers and the volume of each fraction (cells and medium). (D) Corning HYPERStack vessels generated a significantly higher amount of total infectious lentiviral particles compared to stacked vessels of similar cubic footprint.

(A) Final expansion of Human Mesenchymal Stem Cells in Corning HYPERStack 12-layer or control T-flask cell culture vessels with Corning CellBIND® surface treatment using hMSC medium. (B) Total Corning HYPERStack vessel yields of Vero cell cultures were 4X greater than yields from Corning CellSTACK® vessels with similar cubic footprint. (C) Corning HYPERStack vessels generated a significantly higher amount of total infectious adenoviral particles versus stacked vessels of similar cubic footprint. Total infectious adenoviral particles were calculated based on titers and the volume of each fraction (cells and medium). (D) Corning HYPERStack vessels generated a significantly higher amount of total infectious lentiviral particles compared to stacked vessels of similar cubic footprint. *Paired t-test, p <0.05, N=3. Image Credit: Corning Life Sciences

The properties

Corning® HYPERStack®-a closed system for high-yield cell growth

Image Credit: Corning Life Sciences

Table 1. Corning HYPERStack 12-layer vessel. Source: Corning Life Sciences

. .
Surface 6,000 cm2
Volume 1.31 L
Filled weight (approx.) 4.1 kg
Dimensions (L x W) 13.42 x 8.15 inches (342 x 207 mm)
Vessel Height 2.8 inches (71 mm)
Height with Accessory Tray 5.5 inches (140 mm)

 

Corning® HYPERStack®-a closed system for high-yield cell growth

Image Credit: Corning Life Sciences

 

Table 2. Corning HYPERStack 36-layer vessel. Source: Corning Life Sciences

. .
Surface 18,000 cm2
Volume 3.92 L
Filled weight (approx.) 10.8 kg
Dimensions (L x W) 13.42 x 8.15 inches (342 x 207 mm)
Height with Accessory Tray 10.97 inches (278 mm)

 

Corning® HYPERStack®-a closed system for high-yield cell growth

Image Credit: Corning Life Sciences

Ordering information

Table 3. Corning® HYPERStack® Cell Culture Vessels. Source: Corning Life Sciences

Cat. No. Description Growth Area (cm2) Qty/Pk Qty/Cs
20012 Corning HYPERStack 12-layer cell culture vessel, Corning CellBIND® surface, sterile 6,000 1 4
20036 Corning HYPERStack 36-layer cell culture vessel, Corning CellBIND surface, sterile 18,000 1 2
20013 Corning HYPERStack 12-layer cell culture vessel, non-treated, sterile 6,000 1 4
20037 Corning HYPERStack 36-layer cell culture vessel, non-treated, sterile 18,000 1 2

 

Table 4. Accessories. Source: Corning Life Sciences

Cat. No. Description Qty/ Pk Qty/ Cs
431518 2L Erlenmeyer flask with dip tube with 0.2 μm vent, male MPC, chemically resistant, heat sealable flexible tubing, 1/4” ID, 3/8” OD 1 3
11501 5L Erlenmeyer flask with dip tube with 0.2 μm filter, male MPC chemically resistant, heat sealable flexible tubing, ¼" ID, ⅜" OD 1 2
10043 Disposable tubing set for use with 850 cm2 polystyrene roller bottle, 3/8” ID x 1/2” OD, chemically resistant, heat sealable, thermoplastic elastomer tubing, 0.2 µm filter, MPC 1 2
431644 Corning 850 cm2 polystyrene bottle, easy grip cap, non-treated, sterile 1 40
10047 Corning HYPERStack Nest accessory 1 1
6650 Corning Automated Manipulator platform, 180-254 V, standard power supply - -
6651 Corning Automated Manipulator platform, 380-440 V, standard power supply - -
6652 Corning Automated Manipulator cart - -
6655 Corning Automated Manipulator rack for Corning HYPERStack-36, holds six HYPERStack-36 vessels, compatible with Manipulator cart (Cat. No. 6652) - -