Available in 2 formats for analysis:
- TaqMan Probe
Cat. TM39100
- End-Point PCR
Cat. EP39100
Description
Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection.
The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.
There are 2 kits available for the detection of Cryptosporidium:
Cryptosporidium TaqMan PCR Kit
- PCR control to monitor for PCR inhibition and validate the quality
- Master Mix for the target and PCR control detection
- Primer and Probe mix
- Positive control and a negative control to confirm the integrity of the kit reagents
Cryptosporidium End-Point PCR Kit
- Master Mix and primers for the specific amplification of a 218 bp region of the Cryptosporidium genome
- Positive control and a negative control to confirm the integrity of the kit reagents
- Loading dye and a DNA ladder to facilitate analysis of the results
For research use only and NOT intended for in vitro diagnostics.
Specifications
Storage Conditions and Product Stability
All kit components should be stored at -20 °C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20 °C without showing any reduction in performance.
Kit Components
| Component |
TaqMan Probe
Cat. TM39100
(24 rxns) |
End-Point
Cat. EP39100
(24 rxns) |
| MDx 2X RT-PCR Master Mix |
- |
350 µL |
| MDx TaqMan 2X RT-PCR Master Mix |
350 µL |
- |
| Cryptosporidium Primer Mix |
70 µL |
70 µL |
| Cryptosporidium Positive Control |
50 µL |
50 µL |
| Nuclease-Free Water (Negative Control) |
1.25 mL |
1.25 mL |
| Loading Dye |
- |
100 µL |
| DNA Ladder |
- |
100 µL |
| Product Insert |
1 |
1 |
| Component |
TaqMan
Probe/Primer
and Control Set
Cat. TM39110
(100 rxns) |
Primer
and Control Set
Cat. EP39110
(100 rxns)
|
| Cryptosporidium Primer Mix |
3 x 70 µL |
3 x 70 µL |
| Cryptosporidium Positive Control |
3 x 50 µL |
3 x 50 µL |
| Nuclease-Free Water (Negative Control) |
1.25 mL |
1.25 mL |
| Product Insert |
1 |
1 |