Nexcelom Bioscience has introduced a customizable cell counting and image cytometry instrument called Cellometer Spectrum Image Cytometry System.
Introduction to the Cellometer Spectrum Image Cytometer
The following videos show the operation of the Spectrum Image Cytometer:
How Long Does it Take to Set Up a Cellometer Spectrum Image Cytometer?
How to set up the Spectrum.
Cellometer Spectrum Image Cytometer User-Changeable Optics Modules
How to change fluorescent filters.
Features of the Spectrum Image Cytometry System
The Spectrum Image Cytometer — a customizable cell counting and cytometry instrument — offers flow-like data with predesigned templates. Users can rapidly plot cell population data as a histogram, contour plot, scatter plot, or dot plot using FCS express.
The user-changeable filter sets allow users to adapt the instrument for particular reagents or assays of interest.
All-in-One System
The Spectrum Image Cytometer helps perform cell-based assays, primary cell viability, and basic cell counting.
Dual-Fluorescence for Accurate Primary Cell Viability
There is no interference from red blood cells. Users can analyze, cord blood, peripheral blood, and bone marrow without lysing.
Unique Algorithms for Advanced Cell Analysis
Viability and concentration of adipocytes, hepatocytes, and other advanced cell types can be easily determined.
Fast Results
Cell counts, images, viability calculations, and size measurements can be obtained in less than 30 seconds for each sample.
Simple Cell-Based Assays
- Small 20 µL sample size
- Pre-defined instrument settings
- Pre-qualified reagents
- Easy image-based analysis
- Precise and reliable results
- Assay-specific data templates
Advantages of Image Cytometry
Cell Images can be Viewed
- Users can ensure that only the target cells are counted
- Cell morphology can be visually checked
- Images meant for publication can be exported
- Cell images can be archived and re-analyzed
Proprietary Pattern-Recognition Software
Non-Fluidic Platform
- Does not require maintenance
- Disposable counting chambers avoid the need for washing
- Sturdy optics modules and LED light sources
- Can be used with delicate cells
IQ/OQ Validation and GMP/GLP Accessories
- Operational qualification reagents or protocol
- Installation qualification reagents or protocol
- GMP/GLP software module
- On-site OQ or IQ performance
Accurate Cell Counting
Bright field.
Stained AO+ cells.
Counted AO+ cells.
Proven Results
- Cell samples with different viability (0%–100%) can be precisely quantified
- Cell viability can be rapidly determined for primary samples or cell lines using AO/PI
- Up to 2 × 107 cells/mL can be imaged and counted
- Acridine orange (AO)-stained cells (shown above) can be automatically identified
- Versatile platform — can be used for complex cell-based assays and routine cell counting
- Image acquisition and analysis: It takes 30 seconds per sample for a three-channel assay
Bright field.
Stained AO+ cells.
Counted AO+ cells
Export to FCS Express* for Flow-Like Data
Counted PI+ cells.
Contour population plot.
- Simple workflow — Prevents PMT voltages, fluid-stream, and forward/side scatter issues
- Image data can be exported into the flow cytometry software — FCS express
- Cell population data can be rapidly plotted as a histogram, contour plot, dot plot, or scatter plot
- Data analysis can be easily performed using pre-designed templates
Propidium Iodide (PI) Cell Cycle
*FCS Express Flow Cytometry software is a product of De Novo Software and is included with the Cellometer Spectrum.
| Cell Population |
% of Gated Cells |
Concentration (10^6 cells/mL) |
| Total |
100 |
3.18 |
| Sub G1 |
3.8 |
0.12 |
| G0/G1 |
61.9 |
1.97 |
| S |
15.3 |
0.49 |
| G2/M |
19 |
0.60 |
Histogram of PE CD3+ Jurkat cells.
CD3 Bright Field.
CD3 PE.
Contour map of Jurkat cells, showing GFP+ and RFP+ intensity profiles.
Bright field.
GFP/RFP positive cells.
User-Changeable Fluorescence Optics Modules*
Cellometer assays utilize certain optic modules to deliver maximum performance and help differentiate between fluorescence channels. Example filter modules are shown below, but additional filter modules are also available on request.
| Optics Module |
Fluorophores |
Nucleic Acid Stains |
| |
S1-534-470
Ex: 475 nm (BW: 42 nm)
Em: 534 nm (BW: 42 nm) |
GFP
Calcein
FITC
AlexaFluor® 488 |
AO (acridine orange)
SYTO®9, SYTO®13
SYTOX®Green
SYTO®BC |
| |
S1-605-527
Ex: 525 nm (BW: 45 nm)
Em: 605 nm (BW: 64 nm) |
AlexaFluor® 546
AlexaFluor® 555
Cy3®
PE (R-phycoerythrin)
RFP |
PI (propidium iodide)
EB (ethidium bromide)
SYTOX® Orange |
| |
S1-655-527
Ex: 525 nm (BW: 45 nm)
Em: 655 nm (BW: 40 nm) |
RFP
7-AAD
Nile Red |
PI (propidium iodide)
EB (ethidium bromide) |
| |
S1-692-620
Ex: 628 nm (BW: 40 nm)
Em: 692 nm (BW: 40 nm) |
AlexaFluor® 647
APC (allophycocyanin)
Cy5® |
SYTOX® Red |
| |
S1-452-365
Ex: 370 nm (BW: 36 nm)
Em: 452 nm (BW: 45 nm) |
|
Hoechst 33342
DAPI
ViaStain™ Dead Cell Nuclear Blue |
| |
S1-594-470
Ex: 475 nm (BW: 42 nm)
Em: 594 nm (LP – Long Pass) |
|
|
*This table provides a partial list of compatible fluorophores, fluorescent proteins, and nucleic acid stains. Users can contact Nexcelom technical support regarding compatibility of other reagents.