Tecan’s Progesterone Saliva ELISA needs only as low as 50 µL saliva sample volume and has a wide standard range of up to 5,000 pg/mL.
- Method — ELISA
- Regulatory status — EU: CE
- Specimen/volumes — 50 µL saliva
- Kit size — 12 x 8 tests
- Standard range — 25–5,000 pg/mL
- Incubation time — 1 x 1 hour, 1 x 30 minutes
- Substrate/isotope — TMB, 450 nm
The benefit of standardized reagents
Tecan’s Saliva ELISA product line is adapted such that the major steroids (cortisol, testosterone, progesterone, DHEA) may be processed in the same way.
- All kits use the same sample diluent buffer
- The TMB, stop solution and wash solution have similar designs within Testosterone, Cortisol and DHEA saliva ELISA (RE52611, RE52631 and RE52651)
- The kits have six standards and two controls
- The enzyme conjugate, the substrate and stop solutions are pipetted uniformly with 100 µL
- Only 50 µL sample volume is needed
The assays can be effortlessly performed in combination due to the similar processing and assembling.
The two major indications for progesterone measurements are corpus luteum insufficiency and the anovulatorical menstrual cycles in females. It can also be used in anti-aging medications to analyze the progesterone level before and during the replacement therapy.
Repeated saliva sampling is recommended for the pulsatile dynamics of steroid secretion. IBL urges gathering 3–5 saliva samples within 2 hours. Equal volumes of the individual saliva samples can be combined in the lab. The mixed sample provides a mean free Progesterone value, which represents the active hormone concentration in a reproducible way.
Information from the instructions for use
Measurements from this assay can be employed for the diagnosis and treatment of disorders of the placenta or ovaries and can be used as an aid for predicting ovulation. The Progesterone level in saliva shows the concentration of the active free Progesterone.
Progesterone (4-pregnene-3, 20-dione) is a C21 steroid hormone having a keto-group (at C-3) and a double bond between C-4 and C-5. It is produced from cholesterol like other steroids through a series of enzyme-mediated steps.
Progesterone, the steroid hormone, is a female sex hormone that along with estrogens controls the accessory organs at the time of the menstrual cycle. It also plays a significant role in preparing the endometrium for the implantation of the blastocyte and in maintaining pregnancy.
In non-pregnant women, progesterone is majorly produced by the corpus luteum, and in pregnancy, the placenta is the main source. Other minor sources for progesterone include the adrenal cortex for both sexes and the testes for males.