With the Olympus FVMPE-RS, countless possibilities for deep tissue observation are finally realized. The system delivers unmatched high-speed imaging, essential for capturing the dynamic in vivo response, with fine laser control pinpointing the precise site for optimum excitation efficiency - even deep within the sample.
Accompanied by high S/N-ratio imaging and dedicated Olympus multiphoton objectives, efficient detection of scattered fluorescence photons is also ensured. Optimized for reaching greater imaging depths, the system also features simultaneous dual-wavelength excitation (up to 1300 nm).
Visible or multiphoton laser light stimulation and the ability to synchronize with patch clamp data are also possible. In essence, the Olympus FLUOVIEW FVMPE-RS unites high-speed, deep observation capability with multicolor imaging and powerful laser light stimulation, offering a no compromise solution.
High-precision Dual-line Multiphoton Imaging
Multi-wavelength Excitation And Multiphoton Imaging with Precision Imaging Co-registration
Multichannel, multiphoton excitation imaging is accomplished with a dual-wavelength IR pulsed laser or two independent IR pulsed lasers - enabling simultaneous excitation of chromophores with different wavelengths. Thanks to the flexible and precise IR introduction optics both lines are accurately merged. Simultaneous excitation provides perfected registration and balanced images for different chromophores.
InSight DeepSee And Combination with Other Lasers Supports Simultaneous Two Laser Line Excitation and Extended NIR Multiphoton Imaging
The InSight DeepSee pulsed IR laser systems ideally support multiphoton imaging with excitation from 680 - 1300 nm. The Dual-line version of the InSight DeepSee system offers two laser beam outputs: the main output with a tunable line from 680 - 1300 nm and a second output at 1040 nm. Higher laser power beyond 1000 nm provides a host of new multiphoton imaging capabilities, covering a variety of dyes and fluorescence proteins and Third Harmonic Generation imaging without UV damage.
Insight Deepsee. Image data courtesy of: Director Naoki Mochizuki Department of Cell Biology, National Cerebral and Cardiovascular Center
Autocorrect for Laser Beam Misalignment and Pixel Shift with Quadralign 4 Axis Auto Alignment
Multicolor multiphoton laser acquisition provides optimized excitation of different fluorophores, reducing channel crosstalk and photobleaching in the single beam excitation method due to the need to choose a suboptimal middle wavelength for excitation.
To ensure proper colocalization of fluorescent signals, the Quadralign 4 axis auto alignment is incorporated into 2 horizontal and 2 angular axes per laser line, and single-click compensation is also enabled for laser beam position as well as incident laser angle - a common cause of pixel shift by tuning wavelength.
Saving time and effort, this auto alignment mechanism tunes the optical axes of the lasers to the laser wavelength used during multicolor excitation. Software-based fine-tuning is also available.