The GST-Tagged Active HIV1 Protease Recombinant is ≥90% pure active recombinant for inhibitor screening assays, selectivity profiling, activity studies, ELISA, western blotting, and several such applications.
The HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is vital for the life-cycle of HIV. The HIV protease cleaves recently synthesized viral polyproteins at the right places into functional protein products as mature protein parts of an infectious HIV virion. The mutation of HIV protease's active site or hindrance of its activity upsets HIV’s ability to replicate and infect more cells.
||HIV-1 retropepsin, HIV-1 Protease (PR), cd05482
|Physical Form Description
||3 mg/ml in 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol
||38.4 kDa (1-99 aa + N-terminal GST and C-terminal Poly-his tags). It runs at ~31 kDa during SEC and SDS-PAGE analyses.
|Purity by SDS-PAGE
||BioVision’s Active HIV1 Protease has been tested for its activity using HIV1 Protease Activity Assay Kit (K825-100).
|Amino Acid Sequence
||1-99 aa + N-terminal GST and C-terminal Poly-his tags
||Centrifuge the vial prior to opening.
||For research use only! Not to be used in humans