Generation Medium for M1-Macrophages

This defined and xeno-free medium from PromoCell is used for the generation of M1 macrophages from fresh monocytes.


PromoCell Macrophage Generation Media have been made for the efficient generation of monocyte-derived macrophages (MDM), either from newly isolated peripheral blood monocytes or straight from PBMC as a starting material.

The M1-Macrophage Generation Medium DXF has GM-CSF and permits the generation of M1-polarized macrophages. PromoCell’s M1-Macrophage Generation Medium DXF is an instant medium that includes cytokines for the directed differentiation of M1-like polarized MDM.

The Macrophage Media DXF are defined and xeno-free formulation for use with newly isolated cells. Owing to the utilization of completely synthetic, recombinant, or plant-sourced materials, this medium does not contain any animal-derived components and substances of human origin with human serum albumin is the only exemption.


  • Basal Medium M1 (250 ml)
  • CytokineMix M1: GM-CSF (for 250 ml)
  • SupplementMix M1 (for 250 ml)