Large scale imaging overcomes the limits of conventional microscopes with adaptive zoom optics to easily track Zebrafish development from egg to embryo.

The innovative Leica HCS LSI application solution offers the flexibility to efficiently scale up in vivo assays: Aligned acquisition, automation and analysis quickly leads to reproducible, statistically relevant results at minimal effort.

Leica HCS LSI optimally adapts to the experiments and provides 2D, 3D, and 4D screening results.

Increase the efficiency by automation and obtain easily unbiased data for new publications.


High Content Screening in 4D

Maximum information is generated by spatio-temporal Zebrafish analysis. Without being limited by specimen size, Leica HCS LSI offers screening automation from micro to macro scale.

True confocal point scanning technology provides highest resolution. Spectral analysis from 430 to 750 nm optimizes quantum efficiency. High resolution confocal screen and fast digital camera measurement are combined in one system.

Z-positioning methods work for both and  provide precise scans in 2, 3 and 4 dimensions.

Intelligent HCS Automation

Pre-screen and secondary screen are performed on one system running Leica LAS AF Matrix M3 software. Automatically center the embryos and detect rare events when they occur.

Sharp images result from five optimized autofocus modes and the compensation of specimen drift.

Results control the experiment: Trigger the system by image analysis and benefit from the external device control via Computer Aided Microscopy (CAM) interface.

Adaptive Zoom Optics: Discover the Ease

Maximum flexibility by innovative optical zoom technology: Seamless magnification change from overview to finest details with one objective eliminates time consuming adjustment and re-focusing and ensures optimal fit to the area of interest.

In combination with the precise motorized stage, automated operation become facile.

Maximum Flexibility for Assay Development

Maximum automation flexibility by quick operation processes increase laboratory capacities. Profit from the large workspace to get the overview and zoom on the area of interest in seconds.

Track morphological changes, test drugs or identify rare events in Zebrafish. Expand your experiments and adapt the system to other model organisms like Drosophila sp. or C.elegans: Leica HCS LSI offers new perspectives for your research from micro to macro scale.

Balance between image acquisition, automation and analysis parameters to quickly obtain optimal results and reliable data.

Turning Zebrafish into Data

Thousands of images are easily generated. During  the scan the data are immediately streamed to local servers  for analysis. Leica’s OME interface creates the link to existing image analysis solutions  – platform independently. Use your optimized algorithms and save time and effort.

Image analysis controls the experiment: Trigger the system via Computer Aided Microscopy (CAM) interface and automatically detect relevant events.

The Leica Edition of Definiens Developer XD provides additional multi-dimensional analysis, object recognition and report-out functions. The platform is freely programmable. Existing plug-ins can be used and shared.

Efficient Operations for Fast Results

Leica HCS LSI offers freely adjustable acquisition and automation tools for individual experiments up to the final assay.  Perfectly adapt the system to the experiment needs – not vice versa.

Freely apply petri dishes, chamber slides or well plate formats and create individual scanning templates. In the assay development phase, multiple scan jobs are easily assigned to multiple positions to run different experiments on the same well plate, accelerating the upscale process.

Leica HCS LSI provides the flexibility for efficient, automated experiments.