The Cellaca MX high-throughput automated cell counter from Nexcelom Bioscience is an automated cell counter that redefines everything. It allows users to count up to 24 samples in 2.5 minutes using fluorescence, or in 48 seconds using Trypan blue.

Users can streamline their workflow and make it more efficient by integrating the Cellaca MX with these capabilities:

  • Cell lines or primary cells such as PBMCs, tumor digests, and T cells can be analyzed
  • Batch samples are available in 24 well microplates for cell viability and count
  • Robotics can be incorporated with API-compatible instrument
  • Cell integrity can be maintained and sample depletion can be minimized at the time of processing
  • Instrument fluidics no longer have to be maintained

Low-Volume Primary Cell Samples can be Analyzed

The Cellaca MX only requires 25 μL of cells, allowing users to analyze tumor-infiltrating lymphocytes (TILs), mouse splenocytes, tumor biopsy tissue, and much more.

Human PBMC sample stained with Acridine Orange/Propidium iodide (AO/PI) and imaged using bright field and fluorescence on Cellaca MX.

Human PBMC sample stained with Acridine Orange/Propidium iodide (AO/PI) and imaged using bright field and fluorescence on Cellaca MX.

Users can Count Only the Cells of Interest

Brightfield can be used to check viability. Fluorescence can be used when users require particular details on cell health — red blood cells and tissue debris will not artificially modify the count.

Bright field and fluorescent images of PBMCs. In brightfield, white blood cells, platelets, and red blood cells are seen. Nuclear fluorescent staining using both green and red channels accurately identifies only the white blood cells.

Bright field and fluorescent images of PBMCs. In brightfield, white blood cells, platelets, and red blood cells are seen. Nuclear fluorescent staining using both green and red channels accurately identifies only the white blood cells.

User can Count Once and They are Done

The Cellaca MX results are both linear and highly reproducible over a 2-log cell concentration range. Moreover, reliable counts can be obtained across users, laboratories, and systems. In other words, users can run a sample only once and get accurate results in the first attempt itself.

Serial dilution of Jurkat cells from 1.8E+07 to 1.8E+05 cells/mL analyzed on Cellaca MX. Counts were linear across the entire concentration range with CVs < 2.4% for mean cell concentrations of 1.6E+07 cells/mL or higher, and <0.7% for 1.2E+07 cells/mL and below (n = 9).

Serial dilution of Jurkat cells from 1.8E+07 to 1.8E+05 cells/mL analyzed on Cellaca MX. Counts were linear across the entire concentration range with CVs < 2.4% for mean cell concentrations of 1.6E+07 cells/mL or higher, and <0.7% for 1.2E+07 cells/mL and below (n = 9).

The Cellaca MX comes in many different configurations. Each configuration is field-upgradeable, so that users can configure their instrument according to their experimental requirements and rapidly drive to more in-depth cellular insights.

Unlimited Experimental Design

Automated cell counters that need a lot of hands-on time with continuous reloading of slides or those that have the potential to run only in small batches can limit the number of samples to be processed at a time and thus set restrictions on users’ experimental design.

With the Cellaca MX automated cell counter, users can run up to 24 samples at a time in 2.5 minutes or less, enabling them to expand the number of samples that can be counted in just a few seconds.

High Accuracy at Fast Speeds

The ability of the Cellaca MX automated cell counter to reliably image, decluster and report the viability and concentration of cells makes it a multipurpose instrument that can be used in various workflows.

During an experiment, many users over four separate experiments have successfully created sample-to-sample variability for Trypan blue stained CHO cells of below 7% CV with a total of 1,992 samples counted (refer to Figure 1).

High-speed imaging and data reporting of the Cellaca MX automated cell counter enables researchers to finish 83 plates in less than 70 minutes.

Consistent Counting (<7% CV) of Trypan Blue-Stained CHO Cells Over Multiple Experiments

CHO cells stained with Trypan Blue were shown to have a CV of 7% or less over four independent experiments totaling 83, 24-well plates.

Figure 1. CHO cells stained with Trypan Blue were shown to have a CV of 7% or less over four independent experiments totaling 83, 24-well plates.