Similar to the SURFE2R N1, Nanion’s SURFE2R 96SE uses solid-supported membrane (SSM)-based electrophysiology to resolve small currents of pumps and transporters, while increasing the throughput to the next level through the parallelization of 96 measurements.
The transporter samples are added to gold-coated sensors provided in a regular 96 well-plate format. All sensor coating steps are performed by the SURFE2R 96SE itself, thereby minimizing the workload of users.
The transporter can be activated by adding any substrate. The resulting binding or transport currents will be sensed and examined accordingly. The entire process ranging from sample preparation and measurement to data analysis is completely automated.
Key Features of the SURFE2R 96SE
- A 96-channel low-noise amplifier records 96 wells simultaneously
- Automated data analysis and export, for example, determination of EC50
- 10,000 data points per day
- 8 positions for diverse solutions (from 1 well to 96 wells)
- Ability to combine with CyBio® Felix, which is a compact-footprint pipetting platform
- Experimental workflows, including sample preparation, can be automated
Key Features of SSM-based Electrophysiology
- Use purified membranes and vesicles from proteoliposomes or cells
- More than 100 targets confirmed, 100 peer-reviewed papers using SSM-based electrophysiology
- Pumps, transporters, and ligand-gated channels
- Capable of measuring even sugar-binding and electroneutral exchangers
- Needs just 0.1–1 µg protein per sensor, which is adequate for up to 100 experiments
- Quick binding kinetics can be resolved
- Label-free electrical measurements
- Real-time data with a high time resolution of 5 ms, not single-point read-out
- High signal amplification compared to patch-clamp
- IC50, EC50, rate constants, comparison of transporter variants
The technology involves using SSM-based electrophysiology developed in the late 1990s.
For high-throughput environments, the SURFE2R 96SE is preferred over the SURFE2R N1. Such high-throughput settings are necessary in the case of, for instance, compound or transporter screening projects. The SURFE2R 96SE is also ideal to improve throughput for elementary research. When compared to the SURFE2R N1, SURFE2R 96SE ensures 100 times faster data generation.