Human iPSC-Derived Astrocytes

Human iPSC-derived astrocyte kits offered by Axol Bioscience include optimized media, cryopreserved cells, and supplements. These kits provide scientists with a complete, easy-to-use culture system that ensures long-term cell viability for use in drug discovery and disease modeling applications.

Dysfunction of astrocytes has been implicated in several neurological conditions like Parkinson’s disease, Alzheimer’s disease, schizophrenia, Rett syndrome, amyotrophic lateral sclerosis (ALS), and autism.

These kits from Axol Bioscience serve as a physiologically relevant tool for studying the astrocytes in a co-culture with neurons or as an isolated population for the analysis of the central nervous system (CNS), which is challenging.

Human iPSC-derived astrocyte progenitors are engineered to create mature astrocytes within a period of three weeks, wherein prototypical phenotype markers, like S100b and glial fibrillary acidic protein (GFAP), are expressed by iPSC-derived mature astrocytes.

Using Axol’s iPSC-derived mature astrocyte and Human iPSC-derived astrocyte progenitor kits, scientists can gain a deeper understanding of the mechanisms that control drug response, the development of the CNS, and the onset of diseases.

MEA Characterization of Cerebral Cortical Neuron-Astrocyte Co-Culture

Activities of the excitatory neurons — that is, less individual spikes and more bursts — are inhibited and modulated by iPSC-derived cerebral cortical interneurons (ax0667).

iPSC-derived astrocytes (ax0665) offer a physiologically relevant and supportive environment, where the cortical neurons display faster maturation as shown by more frequent spikes, larger spike amplitudes, and increased burst activities.

Calcium Activity

Human iPSC-derived astrocytes from Axol Bioscience were cultured for seven weeks prior to determining the activity of baseline calcium.

Fluo-4-AM (Thermofisher) calcium dye (1:10 dilution) was added to the human iPSC-derived astrocytes and the mixture was incubated at 37 °C for 1 hour. Then, the basal activity was recorded at a rate of 1 fps without stimulus. The video clip below shows 60 seconds of activity that was compressed in 5 seconds for visualization purposes.

Amit K. Chouhan, Abby Scurfield, and Professor Gareth Miles (University of St Andrews) provided the data.