Axol Human iPSC-Derived Sensory Neuron Progenitors derived from a healthy male donor. Axol's induced pluripotent stem cells (iPSCs) were differentiated to sensory neuron progenitors using small molecule inhibitors.
For endpoint assays on plastic, SureBond-XF should be used for coating cell culture surfaces. For endpoint assays on glass, SureBond+ReadySet coating reagent should be used. After thawing and plating, the progenitors need to be growth arrested and then should be matured for approximately 6 weeks prior to endpoint assays.
The expression of Nav1.7 should occur at around 4-5 weeks of maturation and Nav1.8 is expressed after approximately 6-8 weeks of maturation in Sensory Neuron Maintenance Medium.
||Cord blood CD34+ cells
|Donor age at sampling
||A29 A68, B38(Bw4) B44(Bw4), Cw8 Cw12
||Monolayer & chemically defined medium
||1 vial of progenitors (>500,000 cells) and 1 bottle of Neural Plating-XF Medium (30 mL)
||iPSC-derived sensory neuron progenitors