Livecyte Label-Free Cell Analysis System from Phasefocus

Livecyte® is a unique quantitative label-free cell analysis system which delivers continuous tracking and analysis of individual cells within large populations, enabling users to gain a more realistic and comprehensive understanding of cell behavior.

Unlike traditional phase contrast imaging techniques, where images can be corrupted by optical artifacts, Livecyte utilizes ptychography, a QPI (Quantitative Phase Imaging) technique, to generate high contrast, information rich images. This innovative new technology permits the quantitative measurement of phenotypic characteristics and dynamic activity for prolonged periods, permitting multiple cell divisions to be monitored.

Livecyte generates a continuous large field of view independent of imaging objective, eliminating the need for image “stitching”.  As a result, morphological parameters can be reliably determined providing precise and quantifiable metrics. Furthermore a minimal number of cells move beyond the FOV during imaging, allowing even highly motile cells to be accurately tracked over long periods of time.

The system’s low powered laser illumination reduces the risk of phototoxicity and/or photo-induced behavior, ensuring cells remain healthy and viable, making it compatible with sensitive cell types, including primary and stem cells.

This ability to analyze cells in a more natural environment provides reassurance that differences in the behavior of cell populations are genuine rather than a consequence of the imaging technique. validating its suitability for a wide range of applications.

Livecyte also supports widefield fluorescence, permitting labeled and non-labeled protocols to be combined. Supplementing label-free images with periodic tracking of fluorescently labeled components keeps phototoxicity to a minimum, allowing additional information to be extracted and results correlated for confident data analysis.

A further strength of the system lies in its capacity to segment individual cells within complex heterogenous cell populations, enabling multiple parameters to be extracted from a single experiment.

The system comes complete with an extensive array of analysis tools, incorporating both common predefined parameters and customizable options, providing the versatility to tailor assays as required.  With its simple, intuitive workflows and interactive graphical tools, users can rapidly design their experiment, setup their acquisition parameters and analyze their data, all with minimal training.

Livecyte includes 12 TB data storage for simple straightforward data handling, with data exported as graphs, tables images or video. In addition, design, set up and data analysis can all be completed remotely, providing a flexible and efficient resource for multi-user sites.

This comprehensive system has been specifically designed to create an optimal environment for long term live cell assays, heralding a new era in quantitative phase imaging and providing a gateway to the better understanding of cellular processes .

Common applications include but not limited to:

  • Cell migration
  • Mitotic time & cell division
  • Wound healing
  • Angiogenesis
  • Apoptosis
  • Cell proliferation
  • Cytotoxicity & dose response