Tecan’s IBL International Cortisol Saliva Luminescence Immunoassay is fabricated for the in-vitro-diagnostic quantitative determination of Cortisol in human saliva. It can be utilized as an aid in the diagnosis and treatment of adrenal disorders.
- Regulatory status — EU: CE, CDN: IVD
- Method — Luminescence immunoassay
- Kit size — 10 x 12 x 8
- Standard range — 0.015–3.20 µg/dL
- Incubation time — 1 x 3 hours; 1 x 10 minutes
- Substrate/isotope — Luminol
- Specimen/volumes — 20 µL saliva
The luminescence immunoassay is not for point-of-care settings. Cortisol — also called hydrocortisone, compound F — is produced in the zona fasciculata of the adrenal cortex and is the main glucocorticoid in humans.
Around 90% of the circulating cortisol is attached to corticoid binding globulin (CBG, Transcortin), and ca. 7% is attached to albumin and only 1%–3% exists as free cortisol. The latter part represents the active form of cortisol.
The free cortisol is released in saliva and is excreted through the kidneys as a meager part among the metabolites of cortisol. The amount of free cortisol in blood controls its secretion in the adrenal cortex in a negative feedback mechanism through CRH (corticotropin-releasing hormone) in the hypothalamic region and the ACTH in the pituitary gland. However, it is impacted by various situations, above all by stress.
Humans have a physiological fluctuation of cortisol with a peak level in the morning and the lowest during midnight. This fluctuation of cortisol plasma level is seen in saliva generally with a high level in the first 90 minutes after waking up. Adrenal disorders can be identified through cortisol measurement.
The diurnal fluctuations of cortisol make salivary sample collection an effortless method without having to undergo the stress of repeated venipunctures.
Tecan’s IBL Cortisol Saliva Luminescence Immunoassay is calibrated to the reference method of the LC-MS/MS and substitutes the earlier version referenced RE62019. The Luminescence IA can also be procured in regular format (12x8 tests), with the catalog number RE62111. Distributed by IBL International.