Tecan’s Luminescence immunoassay can be used for in vitro diagnostic quantitative measurement of 17beta-Estradiol in human saliva. The results from the instrument can be used for the identification and treatment of different hormonal sexual disorders. However, this test cannot be used for examining placental function in complicated pregnancies.
- Method — Luminescence immunoassay
- Regulatory status — EU: CE
- Standard range — 0.9–64 pg/mL
- Kit size — 12 x 8
- Incubation time — 1 x 4 hours; 1 x 10 minutes
- Substrate/isotope — Acridinium based
- Specimen/volumes — 50 µL saliva
17β-Estradiol [1, 3, 5 (10)-estratriene-3, 17b-diol; E2] is a C18 steroid hormone. It is a significant natural estrogen and is found in males and females. In females, the estrogens induce the growth of sex organs and the formation of secondary sexual characteristics and also impact gonadotropin secretion. In males, the functions of 17β-Estradiol are less defined, but it is involved in regulating the secretion of gonadotropin.
In non-pregnant women, 17β-Estradiol is produced by the ovary; however, after changing into menopause, estrogens are produced by the brain, the liver, the muscles and adipose tissue, too. Measuring 17β-Estradiol is useful to analyze the lack of estrogens, which might be expressed as primary and secondary amenorrhea, delayed puberty and appears during menopause.
For proper interpretation and differential diagnosis, other hormone assays are needed. It is necessary to evaluate the gonadotropin concentration to identify the origin of the lack of estrogens (synthesis or regulation). 17β-Estradiol determination helps in the diagnosis of pubertas praecox in girls and men, its level can be used for the differential diagnosis of gynecomastia.
Around 1%–3% of estradiol circulating in plasma is present in its free form as they are bound to sex hormone-binding globulin (SHBG) and serum albumin. The free form represents the active part in the endocrine regulation and is found in equal volumes in saliva. As the variations of the 17β-Estradiol levels are based on individual situations, it is very easy to obtain a hormone profile by gathering saliva samples repeatedly.