Methyltransferase-like protein 16 (METTL16) available from SignalChem Biotech is an RNA N6-methyltransferase that methylates residues of adenosine at the N6 position of a subset of RNAs.

METTL16 methylates the 3’ UTR of the SAM synthetase (MAT2A) mRNA and U6 snRNA, thereby regulating S-adenosylmethionine homeostasis as well as pre-mRNA splicing.1,2

These modifications on viral RNAs are believed to influence the expression of viral genes, the replication of virus and the generation of progeny virions.3

Recombinant full-length human METTL16 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The Purity of the protein was determined to be approx. 90% through densitometry and the molecular weight is approx. 98 kDa.

The METTL16 activity assay is performed inhouse using the MTase-Glo TM methyltransferase assay.

Product specification

Sample activity

Sample Activity Plot.

Sample Activity Plot. Image Credit: SignalChem Biotech

For specific information on a given lot, users can refer the related technical data sheet.

Specific activity

Purity

 
Sample Purity Data.

Sample Purity Data. Image Credit: SignalChem Biotech

For specific data on a given lot, users can see the related technical data sheet.

Storage, stability, and shipping

The product should be stored at −70 °C. For optimal storage, the target can be aliquoted into smaller amounts following centrifugation and can be stored at the recommended temperature. For best performance, multiple freeze/thaw cycles and repeated handling should be avoided.

Molecular weight

~98 kDa

References

  1. Shima, H. et al: S-Adenosylmethionine synthesis is regulated by selective N(6)-adenosine methylation and mRNA degradation involving METTL16 and YTHDC1. Cell Rep. 2017,21:3354–3363.
  2. Warda, AS. et al: Human METTL16 is a N(6)-methyladenosine (m(6)A) methyltransferase that targets pre-mRNAs and various non-coding RNAs. EMBO Rep. 2017,18:2004–2014.
  3. Dang, W. et al: N6-methyladenosine and viral infection. Front. Microbiol. 2019,10:417.