Recombinant full-length human METTL16 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The Purity of the protein was determined to be approx. 90% through densitometry and the molecular weight is approx. 98 kDa.
Methyltransferase-like protein 16 (METTL16) available from SignalChem Biotech is an RNA N6-methyltransferase that methylates residues of adenosine at the N6 position of a subset of RNAs.
METTL16 methylates the 3’ UTR of the SAM synthetase (MAT2A) mRNA and U6 snRNA, thereby regulating S-adenosylmethionine homeostasis as well as pre-mRNA splicing.1,2
These modifications on viral RNAs are believed to influence the expression of viral genes, the replication of virus and the generation of progeny virions.
The METTL16 activity assay is performed inhouse using the MTase-Glo TM methyltransferase assay.
Sample Activity Plot. Image Credit: SignalChem Biotech
For specific information on a given lot, users can refer the related technical data sheet.
Sample Purity Data. Image Credit: SignalChem Biotech
For specific data on a given lot, users can see the related technical data sheet.
Storage, stability, and shipping
The product should be stored at −70 °C. For optimal storage, the target can be aliquoted into smaller amounts following centrifugation and can be stored at the recommended temperature. For best performance, multiple freeze/thaw cycles and repeated handling should be avoided.
- Shima, H. et al: S-Adenosylmethionine synthesis is regulated by selective N(6)-adenosine methylation and mRNA degradation involving METTL16 and YTHDC1. Cell Rep. 2017,21:3354–3363.
- Warda, AS. et al: Human METTL16 is a N(6)-methyladenosine (m(6)A) methyltransferase that targets pre-mRNAs and various non-coding RNAs. EMBO Rep. 2017,18:2004–2014.
- Dang, W. et al: N6-methyladenosine and viral infection. Front. Microbiol. 2019,10:417.