Microwell Plate-Based Image Cytometer - Celigo Imaging Cytometer

The Celigo Imaging Cytometer available from Nexcelom Bioscience is a micro-well plate-based, bright field and fluorescent imaging system. This bench-top system has been developed for live cell analysis.

  • Data visualization
    • Easy-to-use data visualization environment enables data interpretation
  • Automation
    • Automated handling of microplate for time-point analysis or kinetic end-point analysis
  • Bright field imaging for wells of all sizes
    • The cytometer offers industry-leading bright field imaging for wells of all sizes
  • API and data management
    • Application programming interface (API) and data management solution provided for combining with automated workflows.
  • Bright field and four fluorescent channels

The Celigo Cell Cytometer Counts Every Cell in Every Well

Celigo Imaging Cytometer

 

The Celigo Imaging Cytometer offers industry-leading bright field imaging capability, together with four fluorescence channels. Thus, users can experience high speed, completely automated imaging and measurement of suspension, tumor spheroids, iPSC, adherence cells, and cancer stem cell colonies within 6-, 12-, 24-, 48-, 96-, 384-, 1536-well plates, T flasks, and slides.

Proprietary optics and scanning system allow fast imaging of whole well and maintain consistent illumination and contrast out to the well edge. This enables precise identification of all cells inside each well.

The Celigo Imaging Cytometer includes an intuitive software interface and options to combine with automation platforms, thus offering labs with improved capabilities.

Celigo Imaging Cytometer

 

The Celigo Image Cytometer Provides the Best-in-Class Bright Field Imaging for All Well Sizes

Excellent Optics for Improved Image Quality

Bright field optical image quality at the edge of wells is improved, and edge optical distortion is eliminated using an F-Theta lens for excellent well edge-to-edge image contrast.

Brightfield image taken with Celigo Imaging Cytometer at the edge of a well on a 96-well microplate showing enhanced image quality and contrast.

Brightfield image taken with Celigo Imaging Cytometer at the edge of a well on a 96-well microplate showing enhanced image quality and contrast.

Brightfield image taken with conventional microscope showing distortion and low contrast near the edge of the field-of-view.

Brightfield image taken with conventional microscope showing distortion and low contrast near the edge of the field-of-view.

Example of 12-well plate acquisition using Celigo Imaging Cytometer.

Example of 12-well plate acquisition using Celigo Imaging Cytometer.

Example of 96-well plate acquisition using Celigo Imaging Cytometer.

Example of 96-well plate acquisition using Celigo Imaging Cytometer.

Fast Plate Scanning for Image Acquisition and Analysis

Plate acquisition speed can be drastically increased with the help of innovative plate scanning technology to ensure fast acquisition. A 384-well plate can be scanned in bright field within 2 minutes.

Ability to Scan a Variety of Plate Vessels

Wells of virtually any size can be scanned using automated image stitching that offers the ability to visualize and quantify cells and colonies in vessels up to 6-well plates and 10 cm dishes.

Accurate Quantification of Cells and Colonies

All cells and colonies in the well can be accurately quantified regardless of whether they grow uniformly across the wells.

Brightfield image of a well on a 96-well microplate showing counted cell colonies across the well.

Brightfield image of a well on a 96-well microplate showing counted cell colonies across the well.

Accurate Measurement of Adherent Cells Without Trypsinization

Users can analyze their cell sample without the need for trypsinization. This prevents the loss of cells and enables analyzing cells exactly where they grow over several scan times.

Brightfield image of a well on a 96-well microplate showing counted adherent cells.

Brightfield image of a well on a 96-well microplate showing counted adherent cells.

Bright Field and Four Fluorescent Channels (UV to Far Red) for Analyzing and Quantifying Multiplex Assays

Bright Field and Multi-Channel Fluorescence Imaging

A combination of bright field and fluorescent imaging can be used, thus enabling the multi-color assays from UV to Far Red to be developed.

Fluorescent images acquired with Celigo Imaging Cytometer of (a) Tubulin, Phalloidin, and DAPI, (b) Phospho-ERK and Hoechst, and (c) GFP and mCherry.

Fluorescent images acquired with Celigo Imaging Cytometer of (a) Tubulin, Phalloidin, and DAPI, (b) Phospho-ERK and Hoechst, and (c) GFP and mCherry.

Robust Image Analysis Software

Flow-like data analysis and presentation generated by Celigo Imaging Cytometer. Fluorescence intensity (a) histogram and (b) scatter plot.

Flow-like data analysis and presentation generated by Celigo Imaging Cytometer. Fluorescence intensity (a) histogram and (b) scatter plot.

Clear Visualization of Image and Data Correlation

Color coding overlays can be used to correlate the cell populations detected in gates with the cells viewed in the image.

Correlation of gated scatter plot data to counted cells on acquired images.

Correlation of gated scatter plot data to counted cells on acquired images.

Convenient Data Visualization Environment Facilitates Data Interpretation

Simple At-a-Glance Plate-Based Data Review

Users can quickly evaluate data from one well to another with the help of an at-a-glance view for every single plate.

384-well plate view of wells showing cell confluency on the plate.

384-well plate view of wells showing cell confluency on the plate.

Simple At-a-Glance Plate-Based Growth Curves

Users can produce time course growth curves and present them in a plate overview, thus enabling at-a-glance data review.

Time-course data plot generated by Celigo Imaging Cytometer.

Time-course data plot generated by Celigo Imaging Cytometer.

Convenient Bright Field and Fluorescent Image Navigation

Users can visualize the growth of their cells in particular well locations by navigating between wells, plates, and time points.

Brightfield image of a single cell colony observed at the same location of a well on a 96-well plate.

Brightfield image of a single cell colony observed at the same location of a well on a 96-well plate.

Application Programming Interface (API) and Data Management Solution for Integration into Automated Workflows

Integrate with Robotics

Users can automate their assays by using scheduling software to control the operation of the Celigo, thereby enabling integration with plate stackers, robotic arms, liquid handlers, and automated incubators.

Automated System for High Throughput Integrated Data Acquisition and Analysis

Images and data analysis of hundreds of plates can be automatically acquired 24/7.

Time-Saving Data Processing Method

Simultaneous acquisition and analysis of images enable users to acquire data on the go.

Flexible Data Analysis Method for Multiple Users

The Celigo Satellite Workstation can be used to analyze huge data sets off the Celigo instrument. This feature frees up Celigo time to obtain more number of plates.

Series of Customized Applications for Each Assay — Ready to Use, Does Not Require Any Image Analysis Expertise

Label-Free Bright Field Cell Analysis

Users can leverage label-free bright field applications to prevent cells from being stained with toxic dyes or transfected with fluorescent reporters.

Label-free brightfield cell counting and from (a) low count to (b) high cell count which can be used to directly generate (c) a cell growth curve.

Label-free brightfield cell counting and from (a) low count to (b) high cell count which can be used to directly generate (c) a cell growth curve.

Numerous Cell Characterization Assays

Live cell analysis of images can be performed for cell counting, colonies, confluence and 3D-spheroids.

Label-free brightfield cell counting of (a) spheroids, (b) cell counting, or (c) confluence percentage.

Label-free brightfield cell counting of (a) spheroids, (b) cell counting, or (c) confluence percentage.

Bright Field and Fluorescent Images can be Exported for Publications

Users can capitalize on the use of the bright field and fluorescent image quality offered by Celigo to acquire images of their cells for records and strengthen their publications.

Easy Access and Management of Data Using Celigo Network Database

With the Celigo network database, users can provide access to their imaging data and link several instruments and satellite workstations to easily obtain and analyze their data from a convenient centralized location.

Easy-to-Use Preset Assay Parameters for Fast Data Analysis

Celigo assays can be performed with the help of preset parameters that need researchers to carry out very few modifications.

Simple and Intuitive Software User Interface

Users can take advantage of a simple and intuitive four-step software workflow to run all their assays.

Users can Take Their Analysis to the Next Level, Automation!

Celigo Imaging Cytometer

 

Stacker Automation: No Need to “Baby-Sit” Users’ Plates

By simply integrating the plate stacker to their instrument, users can freely work on other tasks while Celigo performs screening of their stack of plates.

  • Plates are handled with or without lids
  • Capacity of up to 50 plates
  • Transfer rate of 15 seconds between plates
  • Plate formats ranging from 6-wells up to 1536-wells can be accommodated
  • Data is exported automatically for every single plate
  • Can be easily integrated into existing Celigo instruments
  • Can be mounted on a standard lab bench
  • Perfect for endpoint assays
Celigo Imaging Cytometer

 

Parameter Specification
Celigo only Dimensions 19 inch wide x 29 inch long x 17 inch high (with plate holder extended)
Celigo + Stacker Dimensions 25 inch wide x 44 inch long x 40 inch high (Total)
Electrical Power 5 Electrical outlets needed at 100-240 VAC 50/60 Hz (30 Amps Total)
Compressor (sold with Stacker) 1.2 CFM @ 90 PSI with 1 gallon tank
Operating Temperature 15 ºC to 25 ºC
Operating Humidity 10% to 90% RH, non-condensing
Instrument Shipping & Storage Temperature -18 ºC to 65 ºC
Instrument Shipping & Storage Humidity 10% to 90% RH, non-condensing
Manufacturer Nexcelom Bioscience, LLC.
360 Merrimack St., Building 9
Lawrence, MA 01843Kbiosystems Limited
Units 5 to 10 Paycocke Close
Basildon, Essex, UK, SS143HS
Distributor/Support Obtain information at https://www.nexcelom.com/purchase or contact [email protected]

 

 

Celigo plate scan speed.

Celigo key features.