PromoCell provides monocyte-derived, polarized, and non-activated, primary human macrophages. Polarization was performed in M1-Macrophage Generation Medium DXF (including GM-CSF).
Cryopreserved human monocyte-derived macrophages (hMDM) from PromoCell are easy-to-handle and appropriate for laboratory research. The thawed cells plate into all tissue culture vessel setups and can be kept as adherent, biologically functional cultures for several weeks. Optionally, user-tailored activation of the cells can be performed.
Cryopreserved human macrophages are created from monocytes in PromoCell’s well-established M1-Macrophage Generation Medium DXF and are delivered as non-activated, qualified M1- (GM-CSF) polarized cells. The M1 macrophages, after 9-10 days of differentiation, are cryopreserved using PromoCell’s exclusive, defined, and animal-free freezing medium, Cryo-SFM. Each cryo vial has over 1.5 million or 5 million viable cells after thawing, respectively.
PromoCell’s M1 Macrophages are currently also available from HLA-typed donors.
- Recommended plating density: 100,000 cells per cm2
- Tested Markers: CD80 positive, CD68 positive