Monocyte-Derived Human M2 Macrophages (M-CSF)

The monocyte-derived, polarized, non-activated, primary human macrophages are provided by PromoCell. Polarization was performed in M2-Macrophage Generation Medium DXF containing M-CSF.


The cryopreserved human monocyte-derived macrophages (hMDM) from PromoCell are convenient and easy-to-use. The thawed cells plate into all tissue culture vessel setups and can be preserved as adherent, biologically functional cultures for a number of weeks. User-customizable activation of the cells can also be carried out.

Cryopreserved human macrophages are developed from monocytes in PromoCell's well-established M2 Macrophage Generation Media DXF and are delivered as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved with the help of PromoCell’s exclusive, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial has more than over 1.5 million or 5 million viable cells after thawing, respectively.

PromoCell’s M2 Macrophages are also currently available from HLA-typed donors.

Additional Information

  • Suggested plating density: 200,000 cells per cm2
  • Tested markers: CD163 positive, CD68 positive