The monocyte-derived, polarized, non-activated, primary human macrophages are provided by PromoCell. Polarization was performed in M2-Macrophage Generation Medium DXF containing M-CSF.
The cryopreserved human monocyte-derived macrophages (hMDM) from PromoCell are convenient and easy-to-use. The thawed cells plate into all tissue culture vessel setups and can be preserved as adherent, biologically functional cultures for a number of weeks. User-customizable activation of the cells can also be carried out.
Cryopreserved human macrophages are developed from monocytes in PromoCell's well-established M2 Macrophage Generation Media DXF and are delivered as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved with the help of PromoCell’s exclusive, defined and animal-free freezing medium, Cryo-SFM. Each cryo vial has more than over 1.5 million or 5 million viable cells after thawing, respectively.
PromoCell’s M2 Macrophages are also currently available from HLA-typed donors.
- Suggested plating density: 200,000 cells per cm2
- Tested markers: CD163 positive, CD68 positive