Mouse Monoclonal Anti-Alpha Smooth Muscle Actin Antibody from Abcam

Abcam’s mouse monoclonal smooth muscle actin antibody reacts with the smooth muscle cells of blood vessels and the parenchymal tissue of ovary, testis, and intestine.

  • Product Name - Anti-alpha smooth muscle Actin antibody [1A4]
  • Description - Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Host Species - Mouse
  • Specificity - The antibody reacts with the parenchymal tissue of testis, intestine, and ovary, and the smooth muscle cells of blood vessels.
  • Tested Applications - Best suited for ICC, ICC/IF, IHC-P, IHC-Fr, ELISA, WB, and Flow Cyt
  • Species Reactivity - Reacts with mouse, rabbit, rat, pig, sheep, and human. Predicted to work with cow, baboon, and mammals
  • Immunogen - Synthetic peptide corresponding to human alpha smooth muscle Actin (N terminal).

Positive Control

  • WB: HeLa whole cell lysate (ab150035) and nuclear lysate, HEK-293 whole cell lysate
  • FC: SV40LT-SMC
  • IHC-P: Human breast ductal carcinoma tissue
  • ICC-IF: SV40LT-SMC cells

Applications

Abcam’s Abpromise guarantee covers the application of ab7817 in the tested applications listed below. The application notes include suggested starting dilutions; ideal dilutions/concentrations should be established by the end user.

Application Abreviews Notes
ICC/IF ☆☆☆☆☆ Should be used at a concentration of 1–5 µg/mL.
ELISA 1/200–1/1000.
IHC-P ☆☆☆☆☆ Should be used at a concentration of 0.1–0.5 µg/mL. Heat-mediated antigen retrieval can be performed with citrate buffer pH 6 prior to commencing with IHC staining protocol.
IHC-P ☆☆☆☆☆ Should be used at an assay dependent concentration.
WB ☆☆☆☆☆ 1/100 –1/300
Flow Cyt 0.1 µg should be used for 106 cells.
ICC ☆☆☆☆☆ Should be used at an assay dependent concentration.

Target

Function

Actins are extremely conserved proteins that are involved in different types of cell motility and are universally expressed in all eukaryotic cells.

Involvement in Disease

Flaws in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are described by permanent dilation of the thoracic aorta, typically because of degenerative variations in the aortic wall. They are mainly related to a characteristic histologic appearance called “medial necrosis” or “Erdheim cystic medial necrosis” wherein there is loss of smooth muscle cells, degeneration and fragmentation of elastic fibers, and a buildup of basophilic ground substance.

Sequence Similarities

Belongs to the actin group

Cellular Localization

Cytoplasm > cytoskeleton