Mouse Monoclonal Anti-Beta Actin Antibody from Abcam

Abcam’s anti-beta actin antibody is used in loading control and for studying cell motility and eukaryotic cell expression.

  • Product Name - Anti-beta Actin antibody [mAbcam 8226] — loading control
  • Description - Mouse monoclonal [mAbcam 8226] to beta Actin — loading control
  • Host Species - Mouse
  • Tested Applications - The anti-beta Actin antibody is ideal for ICC, ICC/IF, IHC-FrFl, Flow Cyt, IHC-Fr, IHC-P, WB, and IP.
  • Immunogen - Synthetic peptide conjugated to KLH extracted from residues within the 1–100 range of Human beta Actin.
  • Positive Control - ICC/IF, WB, Flow Cyt: Jurkat, HeLa, HEK293, A431, PC12 cells, NIH 3T3. IHC-P: Rat colon (FFPE), Human colon (FFPE)

Specificity

The anti-beta Actin antibody does not cross-react with adult skeletal, smooth, or cardiac muscle actin. This product uses an immunogen that shares 77% homology with Gamma actin/actin cytoplasmic 2. Experimental verification of the cross-reactivity with this protein has not been performed.

Species Reactivity

The anti-beta Actin antibody reacts with rat, mouse, horse, rabbit, cow, chicken, human, dog, zebrafish, pig, Chinese hamster, Armenian hamster, and African green monkey. It is also anticipated to work with sheep and guinea pig.

General Notes

Western Blot Protocol Advice

Abcam recommends blocking with 2%–5% BSA since it has been found that the use of 5% milk considerably minimizes the band intensity for beta actin. If milk block is needed, it is recommended to use ab8224 mouse monoclonal [mAbcam 8224] to beta actin.

Application

The Abpromise guarantee offered by Abcam covers the usage of ab8226 in the following tested applications.  Recommended starting dilutions are included here; the end-user must determine optimal dilutions/concentrations.

Application Abreviews Notes
ICC/IF ☆☆☆☆ A concentration of 5 µg/mL should be used.
ICC ☆☆☆☆☆ Should be used at an assay dependent concentration.
Flow Cyt ☆☆☆☆☆ Should be used at an assay dependent concentration.
The ab170190 Mouse monoclonal IgG1 is ideal for use as an isotype control with this antibody.
IHC-FrFl ☆☆☆☆ Should be used at an assay dependent concentration.
IHC-P ☆☆☆☆ A concentration of 0.1–0.5 µg/mL must be used. Before beginning with IHC staining protocol, heat-mediated antigen retrieval should be carried out with citrate buffer pH 6.
IHC-Fr ☆☆☆☆☆ 1/500
IP ☆☆☆☆☆ 1/100
WB ☆☆☆☆☆ 1/500–1/10000.
The estimated molecular weight is 42 kDa.
The antibody can be blocked with Human beta Actin peptide (ab13772).
Abcam recommends blocking with 2%–5% BSA as it has been found that the use of 5% milk considerably decreases the band intensity for beta actin.

Target

Function

Actins, which are highly preserved proteins that take part in different kinds of cell motility, are universally present in all eukaryotic cells.

Involvement in Disease

Dystonia juvenile-onset (DYTJ) [MIM:607371] is caused due to defects in ACTB. DYTJ is a type of dystonia with juvenile onset. Dystonia is characterized by the presence of sustained involuntary muscle contraction, mostly resulting in abnormal postures. DYTJ patients exhibit generalized, progressive, dopa-unresponsive dystonia, sensory hearing loss, and developmental malformations.

  • Sequence Similarities - The antibody belongs to the actin family.
  • Post-Translational Modifications - The antibody is ISGylated.
  • Cellular Localization - Cytoplasm > cytoskeleton. The antibody is localized in cytoplasmic mRNP granules comprised of untranslated mRNAs.