Mouse Monoclonal Anti-PD1 Antibody from Abcam

Abcam’s mouse monoclonal anti-PD1 antibody recognizes human PD1—a checkpoint protein expressed by activated T and B cells.

  • Product Name - Anti-PD1 antibody [NAT105]
  • Description - Mouse monoclonal [NAT105] to PD1
  • Host Species - Mouse
  • Specificity - This antibody can detect human PD1, a kind of checkpoint protein expressed by activated T and B cells. PD1 is involved in the regulation of immune cell responses.
  • Tested Applications - Ideal for Flow Cyt, WB, ICC/IF, IHC-Fr, IHC-P, and IP
  • Species Reactivity - Reacts with human
  • Immunogen - Cells, tissue, or virus corresponding to Human PD1. In particular, human YT cells (NK-like leukemia cell line) that express PD1.

Positive Control

  • ICC/IF: MOLT-4 cells
  • IHC-P: Human tonsil tissue.
  • MOLT-4 cells. IHC-Fr: Human tonsil tissue
  • Flow Cyt: Human tonsil extract

General Notes

It must be noted that PD-1 is expressed inconsistently in various tissues and that optimization may be needed based on the tissue used for the experiment.

Western Blot Protocol Advice:

Owing to low expression of PD-1, Abcam suggests loading a large amount of sample (100 µg) to identify the band for PD-1. Human tonsil and YT cell line lysates are ideal positive controls. This antibody clone [NAT105] is factory-made by Abcam.

New Alternative Versions of the NAT105 Clone Available:

Applications

Abcam’s Abpromise guarantee covers the usage of ab52587 in the tested applications listed below. Suggested starting dilutions are included here; ideal dilutions/concentrations should be established by the end-user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight is 32 kDa.
Flow Cyt 1/100. ab170190 - Mouse monoclonal IgG1 is ideal for use as an isotype control with this antibody.
IHC-Fr ☆☆☆☆ Should be used at an assay dependent concentration.
ICC/IF A concentration of 5–10 µg/mL should be used.
Abcam recommends Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150117) secondary antibody.
IP Should be used at an assay dependent concentration.
IHC-P ☆☆☆☆☆ 1/50. Heat-mediated antigen retrieval should be performed before initiating with IHC staining protocol.

Target

Involvement in Disease

Genetic variation in PDCD1 is related to the vulnerability to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory, and usually febrile multisystemic disorder of connective tissue. It mainly impacts the skin, kidneys, joints, and serosal membranes. It is believed to signify a failure of the regulatory mechanisms of the autoimmune system.

  • Function - Potential cell death inducer, in association with other factors.
  • Sequence Similarities - Contains one Ig-like V-type (immunoglobulin-like) domain
  • Developmental Stage - Induced at programmed cell death
  • Cellular Localization - Membrane