Mouse Monoclonal Loading Control Anti-GAPDH Antibody from Abcam

Abcam’s anti-GAPDH antibody is used as a loading control antibody. It is a 146 kDa tetramer made up of four 30–40 kDa subunits.

  • Product Name - Anti-GAPDH antibody [6C5] — Loading Control
  • Description - Mouse monoclonal [6C5] to GAPDH — Loading Control
  • Host Species - Mouse
  • Tested Applications - It is best suited for IHC-Fr, ICC/IF, WB, ICC, and ELISA.
  • Immunogen - Rabbit muscle GAPDH
  • Positive Control - ICC/IF: SV40LT-SMC cells, NIH3T3 cells, and HeLa cells

Specificity

Abcam’s GAPDH antibody can be utilized as a loading control antibody. It is a 146 kDa tetramer containing four 30–40 kDa subunits. No cross-reaction occurs between GAPDH and yeast. Initial data signifies that the GAPDH antibody-loading control ab8245 detects the monomer (36 kDa), as well as the dimer forms of GAPDH. However, it does not detect the protein’s tetrameric form.

Species Reactivity

It reacts with human, rat, rabbit, mouse, cat, hamster, chicken, dog, fish, zebrafish, pig, Xenopus laevis, monkey, African green monkey, and baboon. It is anticipated to work with Xenopus tropicalis, guinea pig, and horse, but does not react with cow, goat, and Saccharomyces cerevisiae.

Applications

Abcam’s Abpromise guarantee covers the usage of ab8245 in the tested applications listed in the table below. Recommended starting dilutions have been mentioned; the end-user should determine the ideal concentrations/dilutions.

Application Abreviews Notes
ELISA ☆☆☆☆☆ Should be used at an assay dependent concentration.
ICC Should be used at an assay dependent concentration.
WB ☆☆☆☆☆ 1/500–1/10000.
It detects a band of about 36 kDa (expected molecular weight is 40.2 kDa). PubMed: 16450009.
ICC/IF ☆☆☆☆☆ A concentration of 1–5 µg/mL should be used.
IHC-Fr ☆☆☆☆ 1/500

Target

Function

The GAPDH antibody has glyceraldehyde-3-phosphate dehydrogenase as well as nitrosylase activities, and hence it is involved in glycolysis and nuclear functions, respectively. It also takes part in nuclear events such as RNA transport, transcription, apoptosis, and DNA replication.

Nuclear functions could be the result of the nitrosylase activity that regulates cysteine S-nitrosylation of nuclear target proteins, like HDAC2, SIRT1, and PRKDC (By similarity). In glycolysis, glyceraldehyde-3-phosphate dehydrogenase is considered a major enzyme that catalyzes the preliminary step of the pathway by changing D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Pathway

  • Degradation of carbohydrates
  • Glycolysis
  • Pyruvate from D-glyceraldehyde 3-phosphate: step 1/5