The Capel-205 is a new generation capillary electrophoresis (CE) system from Lumex Instruments, which can detect all classes of analytes in a variety of application areas. This CE system can be an excellent choice not only for R&D and low-scale analyses but also for the customers dealing with numerous samples every day.
Principle of Operation
High-performance capillary electrophoresis is based on the differential migration of components of aqueous samples inside a narrow fused silica capillary worked by an electric field. Separated solutes are quantitatively detected at the capillary outlet by a high-sensitive optical system based on direct or indirect UV absorbance.
Features and Benefits
Comprehensive instrumental options
- Extended autosampler capacity up to 59 positions allows testing numerous samples per months
- Built-in unit for tube caps removal opens samples right before the analysis excluding cross-contamination and evaporation
- New HV block offers voltage up to +/- 30 kV to increase separation efficiency and reduce analysis time
- Redesigned capillary cassette with increased length of the thermostated part of the capillary improves migration time reproducibility
- Spectra scanning enables peak identification
- Wide range of controlled injection pressure allows testing of viscous samples
- Reverse sample injection under vacuum: ultra-short analysis time (less than 1 minute) and sample stacking to reduce detection limit
Exclusive design of the capillary cassettes
- Enlarged thermostatic part of the capillary enhances migration time reproducibility
- Capillary can be easily changed within a few minutes
- Optimized optical scheme decreases the detection limit
Precise liquid temperature control (± 0.1 °C)
- Extended range of applied buffers and improved efficiency in separation
Robust software package Elforun
- Real-time electropherogram visualization
- Combination of instrument control, data acquisition, and data processing
- GLP and GALP compliance
- Electropherogram data processing with overlay mode and standard mathematical operations
- Computation of electrophoresis system parameters
- Wide-ranging data processing algorithm customization
- All types of complex runs are possible including those with pre-programming of variations in analysis conditions
- Customized report, data export to other programs, data exchanges with databases, worksheets, and word processors
Streaming potential control technique
- Improving the repeatability of migration time and analysis accuracy
- Possibility of overnight analyses
Analytical Characteristics
Detectors
- Capel-205 is fitted with a spectrophotometric detector with a wavelength range of 190 - 400 nm
Light source
Tubes
- Standard centrifuge-type 1.5 ml, minimal sample volume 100 µl
Injection modes
- Sample injections by vacuum, pressure, or electrokinetically
- Electrokinetic injection at (+/-) 1 – 30 kV considering time for voltage ramping
- Programmable injection time/pressure/voltage
- Controlled pressure profile of injection with pressure values from -100 mbar to +100 mbar
Analysis
- Current from 0 to 300 µA
- Using a voltage from -30 kV to +30 kV in a 1 kV step and an electronic polarity switch
Equipment and Options
- Capillary electrophoresis system Capel-205
- Elforun software package
- Spare capillary cassettes
- Kits for analysis (by request); the majority of CE-kits of any producers match with Capel-205