Available in 2 formats for analysis:
- TaqMan Probe
Cat. TM32100
- End-Point PCR
Cat. EP32100
Description
Campylobacter jejuni is a curved, rod-shaped and microaerophillic gram negative bacterium. It is one of the most common causal agents of gastroenteritis with diarrhea as the main symptom. While infection of C. jejuni is seldom life-threatening, it is considered one of the most common food-borne bacteria with over 2 million people infected per year in US alone.
Infection of C. jejuni usually results from consumption of poorly prepared food including undercooked meat (particularly poultry), untreated water or raw unpasteurized milk. Traditional identification of C. jejuni involves culturing, however the microaerophilic characteristic of this bacterium make the enrichment process laborious and costly.
There are 2 kits available for the detection of Campylobacter jejuni:
Campylobacter jejuni TaqMan PCR Kit
- PCR control to monitor for PCR inhibition and validate the quality
- Master Mix for the target and PCR control detection
- Primer and Probe mix
- Positive control and a negative control to confirm the integrity of the kit reagents
Campylobacter jejuni End-Point PCR Kit
- Master Mix and primers for the specific amplification of a region of the Campylobacter jejuni genome
- Positive control and a negative control to confirm the integrity of the kit reagents
- Loading dye and a DNA ladder to facilitate analysis of the results
For research use only and NOT intended for in vitro diagnostics.
Specifications
Storage Conditions and Product Stability
All kit components should be stored at -20 °C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20 °C without showing any reduction in performance.
Kit Components
| Component |
TaqMan Probe
Cat. TM32100
(24 rxns) |
End-Point
Cat. EP32100
(24 rxns) |
| MDx 2X PCR Master Mix |
- |
350 µL |
| MDx SYBR 2X PCR Master Mix |
- |
- |
| MDx SYBR 2X PCR Control Master Mix |
- |
- |
| MDx TaqMan 2X PCR Master Mix |
350 µL |
- |
| Campylobacter jejuni Primer Mix |
70 µL |
70 µL |
| Campylobacter jejuni Positive Control |
50 µL |
50 µL |
| Nuclease-Free Water (Negative Control) |
1.25 mL |
1.25 mL |
| Loading Dye |
- |
100 µL |
| DNA Ladder |
- |
100 µL |
| Product Insert |
1 |
1 |
| Component |
TaqMan
Probe/Primer
and Control Set
Cat. TM32110
(100 rxns) |
Primer
and Control Set
Cat. EP32110
(100 rxns) |
| Campylobacter jejuni Primer Mix |
3 x 70 µL |
3 x 70 µL |
| Campylobacter jejuni Positive Control |
3 x 50 µL |
3 x 50 µL |
| Nuclease-Free Water (Negative Control) |
1.25 mL |
1.25 mL |
| Product Insert |
1 |
1 |