Available in 2 formats for analysis:

  • TaqMan Probe
    Cat. TM41300
  • End-Point PCR
    Cat. EP41300

Description

E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7).

In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal.

Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.

There are 2 kits available for the detection of E.coli O157:H7:

Specifications

Storage Conditions and Product Stability

All kit components should be stored at -20 °C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20 °C without showing any reduction in performance.

Kit Components

Component TaqMan Probe
Cat. TM41300
(24 rxns)
End-Point
Cat. EP41300
(24 rxns)
MDx 2X PCR Master Mix - 350 µL
MDx TaqMan 2X PCR Master Mix 350 µL -
E.coli O157:H7 Primer Mix 70 µL 70 µL
E.coli O157:H7 Positive Control 50 µL 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Loading Dye - 100 µL
DNA Ladder - 100 µL
Product Insert 1 1

 

Component TaqMan
Probe/Primer
and Control Set
Cat. TM41310
(100 rxns)
Primer
and Control Set
Cat. EP41310
(100 rxns)
E.coli O157:H7 Primer Mix 3 x 70 µL 3 x 70 µL
E.coli O157:H7 Positive Control 3 x 50 µL 3 x 50 µL
Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL
Product Insert 1 1