For the isolation of genomic DNA from animal tissues, cells, bodily fluids, virus and swabs

  • Isolate genomic DNA from animal tissues, cells, bodily fluids, viruses and swabs
  • Rapid and convenient spin column procedure
  • Purified DNA is of the highest quality and integrity for sensitive downstream applications including PCR, qPCR, genotyping, sequencing and more

Description

This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).

Specifications

Kit Specifications
Column Binding Capacity
25 µg
Average Yield:*
HeLa Cells (1 x 106)
Tissue (from 10 mg kidney)
8 µg
10 µg
Maximum Amount of Starting Material:
Animal Tissues
Cultured Cells
Bodily Fluids (blood, saliva)
Viral Suspension
20 mg
3 x 106 cells
150 µL
150 µL
Time to Complete 10 Purifications
80 minutes

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability

The Proteinase K should be stored at -20 °C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.

Kit Components

Component Cat. 24700 (50 preps) Cat. 24750 (100 preps)
Digestion Buffer A 25 mL 2 x 25 mL
Buffer SK 30 mL 2 x 30 mL
Wash Solution A 18 mL 2 x 18 mL
Elution Buffer B 30 mL 2 x 30 mL
Proteinase K 12 mg 2 x 12 mg
Spin Columns 50 100
Collection Tubes 50 100
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1