Available in 2 formats for analysis:
- TaqMan Probe
Cat. TM45300
- End-Point PCR
Cat. EP45300
Description
Rabies is a viral disease that results in acute encephalitis (inflammation of the brain) in mammals. Rabies is caused by the rabies virus, which is a non-enveloped virus with a single-stranded RNA genome.
The virus is transmitted via saliva, and transmission of the disease almost always occurs as a result of an infected animal biting a non-infected animal. The virus does not survive long outside the host and will remain viable in the carcass of an infected animal for less than 24 hours.
After coming into contact with the virus, rabies will spread through the nerves towards the brain. The virus is slow moving and the average time of incubation from exposure to brain involvement is between 3 to 8 weeks in a dog and 2 to 6 weeks in a cat.
However, incubation periods as long as 6 months have been reported. After the virus reaches the brain, it will then spread to the salivary glands where it can spread through bites. The virus is shed at high levels in saliva.
There is no treatment for the disease, once rabies develops it is fatal. Currently, the only way to diagnose rabies in animals is to submit the brain for post-mortem examinations. Since it is possible to be exposed to rabies without becoming infected, methods for the diagnosis of disease by other methods is urgently required. This will not only help in the prevention of the spread of disease, but may also spare animals that would otherwise be euthanized.
There are 2 kits available for the detection of Rabies:
Rabies TaqMan RT-PCR Kit
- PCR control to monitor for PCR inhibition and validate the quality
- Master Mix for the target and PCR control detection
- Primer and Probe mix
- Positive control and a negative control to confirm the integrity of the kit reagents
Rabies End-Point RT-PCR Kit
- Master Mix and primers for the specific amplification of a region of the Rabies genome
- Positive control and a negative control to confirm the integrity of the kit reagents
- Loading dye and a DNA ladder to facilitate analysis of the results
For research use only and NOT intended for in vitro diagnostics.
Specifications
Storage Conditions and Product Stability
All kit components should be stored at -20 °C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix, Control Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. All reagents can be stored for 1 year at -20 °C without showing any reduction in performance.
Kit Components
| Component |
TaqMan Probe
Cat. TM45300
(24 rxns) |
End-Point
Cat. EP45300
(24 rxns) |
| MDx 2X RT-PCR Master Mix |
- |
350 µL |
| MDx TaqMan 2X RT-PCR Master Mix |
350 µL |
- |
| Rabies Primer Mix |
70 µL |
70 µL |
| Rabies Positive Control |
50 µL |
50 µL |
| Nuclease-Free Water (Negative Control) |
1.25 mL |
1.25 mL |
| Loading Dye |
- |
100 µL |
| DNA Ladder |
- |
100 µL |
| Product Insert |
1 |
1 |
| Component |
TaqMan
Probe/Primer
and Control Set
Cat. TM45310
(100 rxns) |
Primer
and Control Set
Cat. EP45310
(100 rxns) |
| Rabies Primer Mix |
3 x 70 µL |
3 x 70 µL |
| Rabies Positive Control |
3 x 50 µL |
3 x 50 µL |
| Nuclease-Free Water (Negative Control) |
1.25 mL |
1.25 mL |
| Product Insert |
1 |
1 |