Norgen Biotek's Total RNA Purification Kit

For rapid purification of total RNA - including microRNA - without phenol

  • Extract high quality & purity total RNA including miRNA
  • No phenol step required
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Efficiently extract small RNA irrespective of GC content
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Convenient & fast spin column format
  • Isolate from a wide variety of specimens

Description

This kit is suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Specifications

Kit Specifications
Binding Capacity Per Column
Up to 50 μg RNA
Maximum Loading Volume Per Spin Column
650 μL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
20 minutes
Maximum Amount of Starting Material
Liver (10 mg)
30 µg
Kidney (10 mg)
10 µg
Brain (10 mg)
12 µg
Blood (hamster, 100µL
5 µg
HeLa (1 x 106)
15 µg
CHO (1 x 106)
11 µg
Yeast (1 x 108)
30 µg
E. coli (1 x 109)
43 µg

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.

Kit Components

Component Cat. 17200 (50 preps) Cat. 37500 (100 preps)
Buffer RL 40 mL 2 x 40 mL
Wash Solution A 38 mL 2 x 38 mL
Elution Solution A 6 mL 2 x 6 mL
Mini Spin Columns 50 100
Collection Tubes 50 100
Elution Tubes (1.7 mL) 50 100
Product Insert 1 1