Rabbit Polyclonal Anti-GFAP Antibody from Abcam

Abcam’s anti-GFAP antibody is used in the study of cell-specific marker during development of the central nervous system (CNS) to differentiate astrocytes from other glial cells.

  • Product Name - Anti-GFAP antibody
  • Description - Rabbit polyclonal to GFAP
  • Host Species - Rabbit
  • Tested Applications - The Anti-GFAP antibody is ideal for IHC-Fr, IHC-FoFr, ICC/IF, IHC-FrFl, IHC-P, WB, ICC, and IHC-Wholemount.
  • Species Reactivity - The anti-GFAP antibody reacts with rat, mouse, dog, cat, common marmoset, and human. It is also expected to work with pig, cow, and mammals.
  • Immunogen - The anti-GFAP antibody is a recombinant full-length protein corresponding to Human GFAP. Isotype 1 is present in and purified from Escherichia coli.
  • Positive Control - IHC-P: FFPE rat brain normal and FFPE mouse brain normal. WB: Mouse and rat brain and spinal cord lysates.

Specificity

The anti-GFAP antibody particularly distinguishes mammalian GFAP on western blots and immunocytochemically. It detects a band of 55 kDa corresponding to GFAP as well as a GFAP-derived band of 48 kDa. The antibody ab7260 has been effectively used by some customers on zebrafish lysates; however, conflicting data is available to propose that not all batches will be appropriate for studies on zebrafish.

General Notes

In certain cases, the antibody may look red in color. This is because of small amounts of hemolysis and has no effect on the performance of the antibody.

Applications

The Abpromise guarantee provided by Abcam covers the usage of ab7260 in the tested applications listed below. Recommended starting dilutions are included here; the end-user should determine the optimal dilutions/concentrations.

Application Abreviews Notes
IHC-FoFr ☆☆☆☆☆ 1/5000
IHC-Fr ☆☆☆☆☆ 1/500
IHC-FrFl ☆☆☆☆☆ Should be used at an assay dependent concentration.
ICC/IF ☆☆☆☆☆ 1/1000
WB ☆☆☆☆ 1/10000.
It detects a band of about 55 and 48 kDa.
This lower 48 kDa band is considered to be a degradation product.
IHC-P ☆☆☆☆☆ 1/1000–1/2000.
Prior to starting with IHC staining protocol, heat-mediated antigen retrieval should be carried out with a citrate buffer of pH 6.
IHC - Wholemount ☆☆☆☆☆ 1/100
ICC ☆☆☆☆☆ 1/5000

Target

Function

A class-III intermediate filament, GFAP is a cell-specific marker that differentiates astrocytes from other glial cells at the time of development of the central nervous system.

Involvement in Disease

Alexander disease (ALEXD) [MIM:203450], a rare disorder of the central nervous system, is caused due to defects in GFAP. It is a progressive leukoencephalopathy which is characterized by extensive accumulation of Rosenthal fibers - cytoplasmic inclusions in astrocytes.

The most widespread type attacks young children and infants and is marked by progressive failure of central myelination, often causing death typically within the first 10 years. Infants with Alexander disease develop seizures, psychomotor retardation, and leukoencephalopathy with macrocephaly. Patients suffering from juvenile or adult types usually experience bulbar signs, ataxia, and spasticity, as well as a more gradually progressive course.

  • Tissue Specificity - GFAP is expressed in cells that do not have fibronectin.
  • Sequence Similarities - The antibody belongs to the intermediate filament family.
  • Post-Translational Modifications - The antibody is phosphorylated by PKN1.
  • Cellular Localization - The antibody is localized to the cytoplasm and is associated with intermediate filaments.