Rabbit Polyclonal Anti-Histone H3 Antibody from Abcam

Abcam’s Rabbit Polyclonal Anti-Histone H3 antibody has the potential to target a core component of the nucleosome that wraps and compresses DNA into chromatin.

  • Product Name - Anti-Histone H3 antibody—Nuclear Loading Control and ChIP Grade
  • Description - Rabbit polyclonal to Histone H3—Nuclear Loading Control and ChIP Grade
  • Host Species - Rabbit
  • Tested Applications - Ideal for IHC-Fr, IHC-P, Flow Cyt, Dot blot, Electron Microscopy, CHIPseq, ChIP, ICC/IF, IP, ChIP/Chip, WB, ICC, IHC-Wholemoun
  • Immunogen - Synthetic peptide matching Human Histone H3 aa 100 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).

Species Reactivity

The antibody reacts with mouse, dog, rat, human, rainbow trout, chicken, silkworm, zebrafish, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, ferret, Indian muntjac, Schizosaccharomyces pombe, Arabidopsis thaliana, Dictyostelium discoideum, Neurospora crassa, Candida albicans, Trypanosoma cruzi, Toxoplasma gondii, rice, Schistosoma mansoni, and Cyanidioschyzon merolae.

It is predicted to work with a broad variety of other species and mammals.

Positive Control

  • ICC/IF: Methanol fixed HeLa cells.
  • WB: HeLa, Drosophila embryo nuclear extract, NIH/3T3, and S. cerevisiae (Y190) and S. pombe whole cell lysates.
  • ChIP: Chromatin from HeLa cells.

General Notes

The ab171870 Rabbit polyclonal IgG is ideal for use as an isotype control with this antibody. Abcam suggests Goat anti-rabbit IgG H&L (HRP) (ab6721) secondary antibody. Abcam-suggested secondaries are Goat anti-rabbit HRP (ab205718) and Goat anti-rabbit Alexa Fluor® 488 (ab150077).

Applications

Abcam’s Abpromise guarantee covers the usage of ab1791 in the tested applications listed below. Note: Suggested starting dilutions are included here; ideal dilutions/concentrations should be established by the end-user.

Application Abreviews Notes
IHC-Fr ☆☆☆☆☆ 1/200
CHIPseq ☆☆☆☆☆ Should be used at an assay dependent concentration.
Dot blot   1/10000
Flow Cyt ☆☆☆☆ Should be used at an assay dependent concentration.
The ab171870 Rabbit polyclonal IgG is ideal for use as an isotype control with this antibody.
IHC-P ☆☆☆☆☆ 1/100–1/400. Heat-mediated antigen retrieval should be performed before starting with IHC staining protocol.
Electron Microscopy   1/50. Customer feedback
ICC/IF ☆☆☆☆ A concentration of 1 µg/mL should be used.
Methanol fixed cells.
ChIP ☆☆☆☆☆ 2 µg should be used for 106 cells.
IP ☆☆☆☆☆ A concentration of 5 µg/mL should be used.
WB ☆☆☆☆☆ 1/1000—1/5000. The antibody detects a band of about 17 kDa (estimated molecular weight: 15 kDa). It can be blocked with Human Histone H3 peptide (ab12149).
Abcam recommends Goat Anti-Rabbit IgG H&L (HRP) (ab6721) secondary antibody.
ChIP/Chip ☆☆☆☆ Should be used at an assay dependent concentration.
IHC—Wholemount   Should be used at an assay dependent concentration. PubMed: 22219645
ICC ☆☆☆☆☆ 1/100–1/500

 

Target

Function

The Rabbit Polyclonal Anti-Histone H3 antibody is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, restricting DNA accessibility to the cellular machinery, which need DNA as a template.

Histones, thus, play a crucial role in DNA replication, chromosomal stability, DNA repair, and transcription regulation. DNA accessibility is controlled via a complicated set of post-translational alterations of histones, also referred to as histone code, and nucleosome remodeling.

Sequence Similarities

The Rabbit Polyclonal Anti-Histone H3 antibody belongs to the histone H3 group.

Developmental Stage

The antibody is expressed during S phase, following which expression intensely reduces as cell division decelerates down during the process of differentiation.