Rabbit Polyclonal Anti-TNF Alpha Antibody from Abcam

Abcam’s Anti-TNF alpha antibodies can be used in studies focused on inflammatory diseases like psoriatic arthritis.

  • Product Name - Anti-TNF alpha antibody
  • Description - Rabbit polyclonal to TNF alpha
  • Host Species - Rabbit
  • Tested Applications - It is best suited for IHC-FoFr, ICC/IF, WB, neutralizing, IHC-Fr, IHC-P, and ELISA.
  • Immunogen - It is a recombinant full-length protein that corresponds to TNF alpha protein.
  • Positive Control - Purchase matching WB positive control: Recombinant Human TNF alpha protein.
  • Species Reactivity - It reacts with Cynomolgus monkey and human. It is expected to work with non-human primates, monkey, pig, dog, rat, and mouse

Specificity

The immunogen, which is utilized to increase the Anti-TNF alpha antibody, shares 78% homology with rat and mouse TNF alpha protein. Although ab6761 has been successfully used with rat and mouse samples by certain customers, these samples are not subjected to batch test. Therefore, this product is not guaranteed to work reliably with rat and mouse. Abcam, however, recommends ab1793 or ab205587 as an alternative product for use with these kinds of species.

General Notes

Endotoxin content by LAL is less than 10 pg/mL. Abcam recommends Goat Anti-Rabbit Alexa Fluor® 488 (ab150077) and Goat Anti-Rabbit HRP (ab205718) secondaries.

Applications

Abcam’s Abpromise guarantee covers the usage of ab6671 in the tested applications listed in the table below. Recommended starting dilutions have been mentioned; the end-user should determine the ideal concentrations/dilutions.

Application Abreviews Notes
ELISA 1/200–1/1000
IHC-P ☆☆☆☆ 1/100–1/200.
Heat-mediated antigen retrieval should be performed prior to beginning with IHC staining protocol.
IHC-Fr ☆☆☆☆ 1/100–1/200
Neutralizing 1/200
WB ☆☆☆ 1/500—1/2000.
For this product, Abcam recommends membrane blocking with BSA instead of milk.
Ideal for use as a positive control for Western blot against recombinant TNF alpha synthesized in Escherichia Coli.
ICC/IF ☆☆☆☆☆ Should be used at an assay dependent concentration. PubMed: 19458984.
IHC-FoFr Should be used at an assay dependent concentration.

Target

Function

The antibody is a cytokine that binds to TNFRSF1B/TNFBR and TNFRSF1A/TNFR1. Predominantly secreted by macrophages, it can cause cell death of specific tumor cell lines. It is also a powerful pyrogen, which causes fever through direct action or through activation of interleukin-1 secretion. It is even involved in the induction of cachexia. Moreover, it can trigger cell proliferation and promote cell differentiation under specific conditions.

Involvement in Disease

Genetic differences in TNF are responsible for causing susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. This inflammatory, seronegative arthritis is linked with psoriasis. It is also a heterogeneous disorder, which ranges from a subtle, non-destructive disease to an extreme, erosive, progressive arthropathy.

As such, five types of psoriatic arthritis have been defined:

  • Asymmetrical arthritis involving the joints of the extremities
  • Asymmetrical oligoarthritis defined by primary involvement of the small joints of the toes or fingers
  • Arthritis mutilans, a rare but destructive and deforming condition
  • Symmetrical polyarthritis defined by a rheumatoid-like pattern that is likely to involve feet, hands, ankles, and wrists
  • Arthritis of the spine and sacroiliac joints (psoriatic spondylitis)