2019-nCoV RNA-Dependent RNA Polymerase (RdRp)<br /> (Catalog No. C19RP-G241H)

SignalChem's 2019-nCoV RNA-dependent RNA Polymerase (RdRp) was expressed in E.coli cells using a C-terminal His tag.

A novel coronavirus (SARS-CoV-2) has caused a global COVID-19 pandemic of respiratory disease in 2020.1 RNA-dependent RNA polymerase (RdRp) is an enzyme that is crucial to life cycle of RNA viruses including coronaviruses.2

RdRp catalyzes the synthesis of viral RNA, thereby playing a central role in the replication and transcription cycle of COVID-19 viruses, possibly in complex with nsp7 and nsp8.3

Therefore, RdRp is a prime target for nucleotide analog antiviral inhibitors, such as remedesivir, for development of new antiviral therapeutics.4

Product specifications

Activity

Sample Activity Plot.

 Sample Activity Plot. Image Credit: SignalChem Biotech

For specific data on a given lot, users can refer to the related technical data sheet.

Purity

Sample Purity Data.

Sample Purity Data. Image Credit: SignalChem Biotech

For specific data on a given lot, users can refer to the related technical data sheet.

Storage, stability, and shipping

The product should be stored at –70 °C. For optimal storage, the target can be aliquoted into smaller quantities after centrifugation and then stored at the suggested temperature. For best performance, multiple freeze/thaw cycles and frequent handling should be avoided.

Molecular weight

~100 kDa

References

  1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89
  2. Subissi L, et al: One severe acute respiratory syndrome coronavirus protein complex integrates processive RNA polymerase and exonuclease activities. Proc. Natl. Acad. Sci. U.S.A. 2014, 111: E3900–E3909.
  3. Gao Y, et al: Structure of the RNA-dependent RNA polymerase from COVID-19 virus. Science. 2020, 368: 779-782.
  4. Wang M, et al: Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro. Cell Res. 2020, 30:269–271.