SOS® neuronal supplement is part of the LiveLight™ cell culture system encompasses three different photostable products:
- MEMO® medium replaces DMEM
- NEUMO® medium replaces Neurobasal® media
- SOS® supplement replaces B-27® and similar neuronal supplements (e.g. N2, SATO, NS21).
LiveLight™ cell culture products do more than just reduce auto-fluorescence. They actively protect your cells from the lethal effects of photo-induced free radicals that are common components of standard media.
Experiments with cultured cells, including fluorescence microscopy, optogenetics, fluorescence activated cell sorting (FACS), calcium imaging, super-resolution microscopy and automated cell culture, often entail high levels or prolonged exposure to light.
However, cell culture media and supplements contain components that are converted to toxic free radicals by light. In particular, DMEM (Dulbecco’s Modified Eagle Medium) and Neurobasal® medium, as well as cell culture supplements such as B-27®, SATO and NS21, can lead to significant perturbation ofbehavior behaviour and marked increases in cell death. This issue has been discussed, for example, in “Artifacts of Light”, Nature Methods (2013) volume 10 (12) page 1135.
LiveLight™ cell culture media and supplements have been reformulated with specific phototoxic components eliminated and replaced.
LiveLight™ media and supplements allow prolonged exposure of cells to light whilst maintaining high levels of cell viability and functionality. As shown below, this allows the generation of remarkably improved experimental data.
7-day old primary rat neuronal cells cultured in standard media or LiveLight™ media in dark and light conditions. Cells were stained with neuronal markers Tuj1 and GFAP, and with DAPI, a marker of cell viability. Light conditions result in widespread cell death with standard media whereas cells cultured in LiveLight™ media show no decrease in viability.
Successful FACS sorting, culturing and differentiation of viable GFP and RFP-labeled central nervous system stem cells (OPCs) from adult mouse brain. (A) Neurobasal® and B-27® destroy cell viability (B) Cells maintained in NEUMO® and SOS® retain viability. Data courtesy of Abbe Crawford and Prof. Robin Franklin of the Wellcome Trust-MRC Cambridge Stem Cell Institute. Scale bar = 100 mm.
OPCs (A,B),Cortical Neurons (C) and Hippocampal Neurons (D) grownin standard conditions and in LiveLight™ media.