Sensitive Fluorometric Assay for HIV-1 Protease Activity Detection

The Human Immunodeficiency Virus (HIV) causes the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is crucial for the life-cycle of the virus as it cleaves recently synthesized polyproteins at the right places to form the mature protein components of an infectious HIV virion. Without effective HIV1 protease, HIV virions stay non-infectious.

The HIV-1 Activity assay kit from BioVision utilizes the capacity of active HIV-1 protease to cleave a synthetic peptide substrate to discharge the free fluorophore which can be quantified (Ex/Em = 330/450 nm) using a fluorescence microplate reader or fluorometer without difficulty. This assay kit is simple, fast, and capable of detecting HIV-1 protease activity in samples of purified enzyme.

Product Details

Cat # +Size K825-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 330/450 nm)
Applications Detect activity of recombinant HIV-1 protease
Determine activity of purified HIV-1 protease
Features & Benefits Fluorometric detection
Kit Components HIV-1 Protease Assay Buffer
HIV-1 Protease Dilution Buffer
HIV-1 Protease Substrate
Fl. Standard (10 mM in DMSO)
Storage Conditions -80 °C
Shipping Conditions Dry Ice
Usage For research use only! Not for use in humans