Reducing Time to Maturation by Over 40 Days!
Significantly Increased Marker Expression
An average increase of 30-45% for neurons expressing TRPv1, Nav1.7 and Nav1.8
Faster Maturation, Quicker Results
- Reducing time to maturation by OVER 40 DAYS
- 10 hours less labour
- Significantly reduced infection potential through extended culture
- 15 less half-media changes
- 2 less full-media changes
MATURATION MAXIMIZER is a fully defined media supplement to enhance the existing sensory neuron maintenance medium (ax0060) and works by mimicking in-vitro, the cell to cell communication and interaction factors which occur when these cells are in their innate environment. The supplement is known to contain factors present in the peripheral nervous system and in particular the peripheral environment of sensory neurons.
New supplemented mature sensory neurons carry the same activation, and to an extent the expression state, of sensory neurons in-vivo, such as the expression markers TRPv1, TRPa1, TRPv2, TRPv3 and TRPm8
Kinetic states of i.e.TRPv1 are in a fast response inward rectifying state, to such antagonists as Capsaicin. Utilizing this new system also encourages faster development and an earlier onset of maturity.
Significantly Enhanced Marker Expression
Reducing maturation time by over 40 days!
The MATURATION MAXIMIZER supplement encourages a more physiological relevant model with neuronal response to capsaicin, occurring at 1uM for 90% of the sensory neuron population.
||1 x 250 mL bottle of Sensory Neuron Maintenance Medium (ax0060) and 4 x 250 uL vials of MATURATION MAXIMIZER supplement
||vapour phase nitrogen
||The medium requires supplementation with Glial-Derived Neurotrophic Factor (GDNF), Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF) and Neurotrophin-3 (NT-3)